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The papers to the solvent extraction method, two-phase solvent extraction the Ganoderma fruiting body applanatum [Ganoderma applanatum (Pers.) Pat] chemical composition of the extract; while isolated groups divided into test substances on chemical liver Injury and inflammatory and analgesic effects research; chemical methods of thin-layer chromatography (TLC), silica gel column chromatography, Sephadex LH-20 column chromatography petroleum ether extract of the active ingredient, acetic The ester extracts the separation, purification, identification. First, the use of solvent impregnation method to extract the organic compounds in the fruiting bodies of Ganoderma lucidum applanatum. Tree tongue dry fruiting bodies of Ganoderma lucidum (40 ° C drying), crushed, placed in a stainless steel bucket with lid, 3 petroleum ether at room temperature extraction, merging three extracts, petroleum ether extract concentrated under reduced pressure. Tree tongue the Ganoderma coarse waving to ether flavor, then with 95% ethanol extraction method with petroleum ether. The obtained ethanol extract. The ethanol extract was dispersed in water, washed successively with ethyl acetate and n-butanol extraction, the ethyl acetate extract, and n-butanol extracts were obtained. The traditional polysaccharide extraction method (water extraction and alcohol precipitation) to extract the fruiting bodies of Ganoderma lucidum polysaccharides applanatum in addition to protein after Sevage law, the application of phenol - sulfuric acid method its total sugar content was measured, and the results show that the extract of total The sugar content of 57.1%. Using ethanol extraction, the alkali extraction acidification extraction applanatum Ganoderma acidic component. Second, the application of chemical-induced liver injury model induced by carbon tetrachloride, a preliminary study of tree tongue Ganoderma petroleum ether extract, ethyl acetate extract, n-butanol extract, polysaccharides, acidic components of the protective effect of the chemical liver injury . The results showed that the petroleum ether extract (0.1 g / kg), ethyl acetate extract (0.2 g / kg) and crude polysaccharide (0.4 g / kg) could inhibit experimental liver injury in mice ALT and AST activities (P < 0.05, P <0.01), prompted a protective effect on the liver. Petroleum ether extract with increasing dose, ALT, AST values ??tended to increase, and large doses of tree tongue Ganoderma petroleum ether extract of the liver toxicity. N-butanol extract similar dose and the role of the acidic components, not to see the obvious hepatoprotective effect. Xylene-induced mouse ear swelling, writhing, hot plate method to study the five kinds of applanatum Ganoderma extract anti-inflammatory and analgesic activity. The results show that, Ganoderma lucidum petroleum ether extract of applanatum, n-butanol extract crude polysaccharide inflammation induced by xylene significant anti-inflammatory effect, which butanol extract the best mouse ear induced by xylene profile of swelling inhibition rate was 30.2%; the crude polysaccharide For the pain caused by the chemical stimulation (volume fraction of 0.6% acetic acid) has a significant analgesic effect, can effectively reduce due to acetic acid-induced writhing number of reactions, analgesic percentage of 37.2%. For physical stimuli (hot plate method) due to pain, crude polysaccharides to take a long the metabolic time to play significant analgesic effect, significantly prolonged mouse hot plate pain threshold, the crude polysaccharide analgesic effect than aspirin analgesic role. The ethyl acetate extract of the acidic component does not exhibit anti-inflammatory and analgesic activity. Finally, the chemical method of thin layer chromatography (TLC), column chromatography on silica gel, dextran gel LH-20 column chromatography, etc., of petroleum ether extract, ethyl acetate extract separated and identified. The petroleum ether extract was purified by column chromatography on silica gel, eluting with petroleum ether - ethyl acetate system, repeatedly recrystallized to give compound 2, and then by Dextran LH-20 column chromatography, eluting with ethyl acetate to give Compound 1. The ethyl acetate layer was subjected to silica gel column chromatography, was eluted with chloroform - methanol system repeatedly, and then by Dextran LH-20 column chromatography with methanol as an eluent to give compound 3. The analysis and identification of the compound manager of detectable, TLC, NMR spectroscopy, mass spectrometry and other methods and literature identified compounds 1-2 were unsaturated fatty alcohols, ergosterol, Compound 3 to be further identified structure.
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