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In this study, lavender leaves as explants established English lavender callus regeneration system ; and callus receptors by Agrobacterium tumefaciens were Vgb ( Vitreoscilla hemoglobin gene ) gene genetic transformation, to obtain regenerated plants . Lavender gene transfer Vgb on physiological and biochemical characteristics were identified. The main results of this study are as follows: MS 2,4- D 0.10 mg / L KT 0.30 mg / L for callus induction medium ; pale yellow callus subculture after 2 or 3 times in MS 6- BA 2.0 mg / L NAA 0.50 mg / L medium cultured for 30 days after the formation of a large number of multiple shoots . Subject to 1-2 cm after small bud into MS 6-BA 1.0 mg / L propagation propagation medium 1 to 2 generations. Propagation , the little buds into 1/2 MS IAA 1.0 mg / L medium for rooting , 20 d after rooting rate 93.33% , to obtain a complete plant regeneration . 2 Agrobacterium lavender callus, get turn Vgb transgenic plants . Conversion conditions: no pretreated callus directly in the broth OD value of 0.4-0.8 after 15 min immersion dark culture 24 h. After treatment , the callus is transferred to add 30 mg / L Kan and 300 mg / L cefotaxime medium transgenic callus induction of differentiation , while obtaining regenerated plants . Step detected by PCR screening of transgenic plants , the positive plants transplanted , the survival rate of 100% or more. 3 pairs of transgenic and non-transgenic lavender lavender for peroxidase, chlorophyll content, proline , soluble sugar, soluble protein Physiological comparison . The results showed that: Lavender in transgenic oxide enzymes, chlorophyll content, proline , soluble sugar, soluble protein content were higher than non-transgenic cause. 4 waterlogging waterlogging treatment test indirectly indicate gene was expressed .
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