|
In this study, yellow flowers and sweet-scented osmanthus (Melilotus officinalis L.) as test material, activity tracking method, were isolated and purified antimicrobial active ingredient of yellow flowers and sweet-scented osmanthus, the activity was measured as well as the structural identification. The results are as follows: 1. Growth rate method and potted test method to test the antibacterial activity of the extracts of yellow flowers and sweet-scented osmanthus. The results show that, the yellow Melilot antibacterial active ingredient is mainly concentrated in the ethyl acetate extract. Dry sample, 5 g / L concentration, ethyl acetate extract on Sclerotinia sclerotiorum, corn leaf spot pathogen Alternaria brassicae have good inhibitory activity, inhibition rates were 93.6%, 89.9% and 84.9% ethyl acetate extract material and the chloroform extract of wheat powdery mildew and wheat stripe rust have a higher protective effect; pot experiment results show that the dry sample 10 g / L concentration, control effects were 73.4% and 63.3% and 58.6% and 60.1%, respectively. Used in vitro method and circular paper chromatography with pre-trial and pre-test the chemical composition of yellow flowers and sweet-scented osmanthus. The results showed that Melilotus suaveolens might contain volatile oils, fats, steroids, terpenes, amino acids, polypeptides, proteins, lactone, coumarin and its glycosides, sugar and their glycosides such as chemical composition and is not detected alkaloids flavonoids and other substances. The Sclerotinia sclerotiorum tracking bacteria, antimicrobial active ingredient of yellow flowers and sweet-scented osmanthus separation. 4 active compounds were isolated from the ethanol extract of yellow flowers and sweet-scented osmanthus, which, the monomer compounds R 3 not playing due to the small number of spectra identified the other three compounds were identified as 2 - benzopyranone acid, palmitic acid and beta-valley sterol. 4 Determination of the Antimicrobial Activity of 1,2 - benzo-pyrone. The growth rate method for the determination of 1,2 - benzopyrones Sclerotinia sclerotiorum, Botrytis cinerea, Rhizoctonia solani, Fusarium graminearum, Alternaria solani and cucumber anthracnose pathogen virulence, the EC 50 2.2 mg / L, 14.1 mg / L and 10.7 mg / L and 31.8 mg / L and 27.6 mg / L and 45.7 mg / L. 1,2 - benzopyrones cucumber anthracnose fungus, Fusarium graminearum, Botrytis cinerea, corn leaf spot pathogen, Bipolaris maydis and rice blast fungus spore germination inhibition poison to inhibit spore germination was determined force, the EC of 50 57.3 mg / L and 64.8 mg / L, 192.6 mg / L, 9.9 mg / L, 94.5 mg / L and 8.9 mg / L; determination of 1,2 - benzopyrones wheat stripe rust in vivo antibacterial activity. Dose of 1000 mg / L, l, 2 - benzopyrones the protective effect of wheat stripe rust was 57.9%, the treatment effect was 40.9%.
|