Dissertation > Excellent graduate degree dissertation topics show

The Preliminary Functional Analysis of Px26 Gene of Plutella Xylostella Granulovirus

Author: ZhangYaQin
Tutor: LiLuLin
School: Central China Normal University
Course: Biochemistry and Molecular Biology
Keywords: baculovirus Plutella xylostella granulovirus membrane fusion protein GP64 F protein
CLC: S476.15
Type: Master's thesis
Year: 2009
Downloads: 35
Quote: 0
Read: Download Dissertation

Abstract


Baculoviruses are classified as a group of arthropod-specific viruses with circular, double-stranded DNA.Envelope fusion protein of the viruses is a major structural protein,which can mediate the entry of virus and may affect the host range.The BVs(budded virions) of baculovirus have two different types of envelope fusion proteins:GP64 and F protein.GP64 is essential for groupⅠNPV BV,which can mediate membrane fusion under the condition of low-pH-triggered and is necessary for efficient virion budding from the cell surface.The envelope fusion protein encoded by groupⅡNPV and GV is named F protein,such as LD130,SE8 and AgseF.But the envelope protein encoded by groupⅠNPV and homolog of the F protein is named F homologous protein,such as AC23 and OP21.Like GP64,F protein of groupⅡNPV and AgseGV possess pH-triggered membrane fusion activity,and mainly located on the cell membrane of the cells transfected.Plutella xylostella granulovirus(PlxyGV) is mainly pathogenic to the diamondback moth.Like groupⅡNPVs and AgseGV,there is not a homolog of gp64 gene in PlxyGV genome.But it is found that orf26(Px26) of PlxyGV was homologous to AgseGV f gene with high sequence similarity.A recent research report indicated that AgseGV f gene could functionally substitute gp64 of Autographa californica multiple nucleopolyhedrovirus(AcMNPV).Thus,in order to detect the functional attribute and the location of the predicted encoding product of Px26,transient expression plasmid of Px26,fused with a copy of the gene of enhanced green fluorescent protein(EGFP),was separately transfected into some insect cell lines.It was shown that Px26-EGFP mainly positioned on the cell membrane of cells transfected with the transient expression plasmid of the fused gene Px26-egfp,but cell membrane fusion was not observed.Lamda-Red recombination system was mainly used for the study of the genome of E.coli.In recent years,with the development of baculovirus shuttle vector,this kind of recombinant system has been become a powerful tool for identifying gene function of baculovirus.The second part of this paper is to knock out gp64 of AcMNPV Bacmid by use of Lamda-Red recombination system and the analysis of transfection-infection, obtaining the recombinant bacmid.It will lay the basis of detecting the Px26 gene and identifying other exogenous gene function.The work is under way.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Recovery Whey Soy Protein by Microfiltration and Development of Whey Powder,TQ936.2
  3. Research on Disease Related miRNA Mining Based on Protein-Protein Interaction Network,R341
  4. The Research and Implementation of Protein Classification Algorithm on the Basic of String Kernel,TP301.6
  5. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  6. Expression of hBMP4 and hBMP7 in Chinese Hamster Ovary Cells,Q78
  7. The Study of Color Change and Control Technology for Oyster Protein Beverage,TS254.4
  8. The Value of the Heart-Type Fatty Acid Binding Protein in Early Diagnosis of Acute Myocardial Infarction,R542.22
  9. Serum A-FABP Levels and Its Expression in Atherosclerotic Plaques of Diabetes Mellitus,R587.1
  10. Impact on Drosophila Brain Developement Through the Overexpression and Loss of Function of Gsα,Q75
  11. Study on Deodorization Techniques of Oyster Protein Beverage,TS254.4
  12. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  13. Study on Preparation and Properties of Protein Isolates from Tilapia,TS254.4
  14. Expression and Purification of Influenza a Virus M2 Protein and Detection of Its Antigenicity,R392
  15. Broiler Responses to Reduced-protein Diets Suppleme Nted with Synthetic Amino Acids and Small Peptides,S831.5
  16. The Preparation of Hpv58e7 Protein by GST-tagged Prokaryotic Expression System,R392
  17. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  18. Comparative Study on Reproductive Biological Characteristics of Helicoverpa Armigera and Helicoverpa Assulta (Lepidoptera:Nuctuidae),S433
  19. Effects of Low-protein Diet on Production Performance and Feeding Environment of Piglet,S828.5
  20. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  21. Identification of B.melitensis 16M Protein Immunodominant Antigens,S852.61

CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control > The use of microbial pathogens > Nematodes
© 2012 www.DissertationTopic.Net  Mobile