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High performance capillary electrophoresis (HPCE) since the 1980s, the rapid development of a new type of separation technology, it has a high separation efficiency, high analysis speed, high sensitivity, low sample consumption and low cost advantages, such as life science, drug analysis, environmental science and food safety testing is playing an increasingly important role. This paper by capillary electrophoresis with UV detection respectively five kinds quinolone antibiotics (gatifloxacin, lomefloxacin, enoxacin, ciprofloxacin, ofloxacin), four kinds of common flavonoids (rutin, kaempferol, luteolin and quercetin), four kinds of natural antioxidants (daidzein, genistein, apigenin and catechin) for separation and detection. Emphasis on the type and concentration of the buffer solution, pH, separation voltage and injection time of the separation results to determine the optimal separation conditions. Five kinds of quinolone antibiotics in the most optimal separation conditions, 9 min to achieve baseline separation. Migration time and peak height relative standard deviation, respectively: 0.78% -0.87% 3.03% -5:67% among and between; 5 kinds of substances in concentrations and electrophoretic peak height at 1 × 10 -5 < / sup> - 1 × 10 -4 mol · L -1 range with a good linear relationship; with three times the peak height of the noise value (S / N = 3 ) to determine the detection limit is: gatifloxacin, ofloxacin, lomefloxacin and enoxacin (4 × 10 -6 mol · L -1 ), ciprofloxacin (2 × 10 -6 mol · L -1 ). The method was applied to the detection commercially available antibiotic drugs lomefloxacin tablets active ingredient content determination, recovery was 109.46%. Four kinds of flavonoids in the most optimal separation conditions, 7 min to achieve a baseline separation. Migration time and peak height relative standard deviation, respectively: 0.65% -0.96% -8.38% among and between 7.1%; 4 kinds of concentration of a substance with the electrophoretic peak height, respectively 1 × 10 -5 - 5 × 10 -4 mol · L -1 range with a good linear relationship; with three times the peak height of the noise value (S / N = 3) determine the detection limit is: rutin and luteolin (2 × 10 -6 mol · L -1 ), kaempferol and quercetin (5 × 10 < sup> -6 mol · L -1 ). While the Chinese Dodder and Zhenjujiangya tablets active ingredient determination, Chinese Dodder rutin content of 2.15 mg · g -1 , kaempferol content was 3.75 mg · g -1 , quercetin content was 4.32 mg · g -1 . Zhenjujiangya film rutin content of 65.5 mg · g -1 . Dodder rutin, kaempferol and quercetin recoveries were 94.2%, 107.8% and 102.8%, rutin Zhenjujiangya film recoveries of 106.2%. Five substances in the most optimal separation conditions, 6 min to achieve baseline separation. Migration time and peak height, respectively relative standard deviation: 0.7% -0.8% and -7.9%, and between 3.9%; five components concentration and peak height, respectively electrophoretic 1 × 10 -5 - 2 × 10 -4 mol · L -1 and 2.5 × 10 -5 - 5 × 10 -4 < / sup> mol · L -1 range with a good linear relationship; with three times the peak height of the noise value (S / N = 3) to determine the detection limit of: daidzein and genistein Su (2 × 10 -6 mol · L -1 ), apigenin and its impurities and catechin (5 × 10 -6 mol · L -1 ). daidzein, genistein, apigenin impurities, apigenin and catechin recoveries were 95.3%, 106.8%, 102.6%, 97.6% and 104.1% The method can be developed on the actual separation of the sample application.
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