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Study on the Contents of Polyphenol, Flavonoid and Antioxidant Activity in Vitro of Houttuynia Cordata Thunb.

Author: CaiWenGuo
Tutor: WuWei
School: Sichuan Agricultural University
Course: Pharmaceutical Botany
Keywords: Houttuynia Total phenolic Folin-Ciocalteu method Flavonoids DPPH Anti- β -carotene bleaching capacity ABTS Position Populations
CLC: S567.239
Type: Master's thesis
Year: 2011
Downloads: 252
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Abstract

Houttuynia is commonly used in traditional Chinese medicine, the original plant Saururaceae plant Houttuynia (Houttuynia cordata Thunb.), Is determined by the Ministry of Health official medicinal and edible plant resources. Since ancient times, people's favorite features are Houttuynia vegetables. Polyphenols are widely present in plants, can affect food flavor (polyphenols Pickle, astringent), is also associated with health functions are closely related. The polyphenols is flavonoids and antioxidant, anti-cardiovascular diseases, anti-tumor activity of closely related organisms, and is important in the future of natural dyes. Sichuan is rich in Houttuynia resources in the wild by the formation of long-term evolution of different ecological types (populations) of Houttuynia polyphenols and flavonoids and antioxidant activity of comparison, the development and utilization of Houttuynia resources, breeding high nutritional value and good taste varieties suitable for food Houttuynia guiding significance. In this study, total phenol extraction Houttuynia and determination methods are discussed, as well as comparison of different sources in different parts of Houttuynia Houttuynia materials total leaf phenolics and flavonoids and their respective in vitro antioxidant activity. The main results are as follows: 1. Use of ethanol, methanol, acetone and water, ultrasonic extraction of four different solvents Houttuynia leaves extracts compare different time Folin-Ciocalteu method using the absorbance values ??measured. 2h and ethanol extracts of the type of the object of sodium carbonate solution, Folin-Ciocalteu reagent was added, reaction temperature and time, size, and the absorbance of the extract solution stability. The results showed that the highest extraction efficiency of methanol, followed by ethanol, acetone and water; ideal extraction time is 2h. Suitable measurement method for the introduction of 0.5 mL extract, followed by adding 2.0 mL 20% sodium carbonate buffer and 1.5 mL Folin-Ciocalteu reagent, distilled water volume to 50.0mL. Incubated at 55 ℃ 1.5 h, measured at a wavelength λ = 760nm absorbance. This method is fast, accurate and stable, suitable for promotion. (2) selection W01-100 and W01-94 Houttuynia two materials were measured its leaves, flowers, stems and roots of four different parts of total phenols, flavonoids and other content, and the DPPH (1,1 - diphenyl picrylhydrazyl) values, anti-p-carotene bleaching ability and ABTS (2,2 '- azino - bis - (3 - ethyl-dihydro-benzo-thiazole-6 - sulfonic acid)) values, etc. Antioxidative activity indicators. The results showed that different parts of Houttuynia total phenolic content ranged from 1.90-10.26mg gallic acid g-1 dw; flavonoid content ranged from 0.751 ~ 12.4 mg rutin g-1 dw; DPPH values ??ranged from 84.7 ~ 248μM trolox g- 1 dw; anti-β-carotene bleaching capacity size between 19.62 ~ 88.62%; ABTS values ??in the range 78.4 ~ 218μM trolox g-1 dw between. In vitro antioxidant activity (DPPH values, anti-β-carotene bleaching ability and ABTS values) and total phenolics and total flavonoids same trend in the performance of each organ: leaf flower = gt; stem gt; roots. The two tested materials from various parts of the total phenolics and antioxidant activity in vitro showed no significant difference. In view of different parts of Houttuynia total phenolics and antioxidant activity in vitro differences, it is recommended to use different parts of Houttuynia respectively for biological activity evaluation. 3.16 agronomic characters were good Houttuynia materials (including a copy Emei Houttuynia cordata) leaf total phenols and flavonoids and antioxidant activity in vitro there were rich variation. The total phenolic content of 7.01 ~ 15.0 mg gallic acid g-1 dw changes between; total flavonoid content ranged from 3.56 ~ 11.0 mg gallic acid g-1 dw; DPPH value of the minimum 84.7μM trolox g-1 dw, up to 248μM trolox g-1 dw; while the ABTS ranged 78.4 ~ 218μM trolox g-1 dw. Emei Houttuynia regardless of which total phenol, total flavonoid content and in vitro antioxidant activity was lower. Houttuynia materials chromosome number and total phenolic content and antioxidant activity in vitro is not associated with significant, while total phenols, flavonoids and antioxidant activity in vitro correlation between indicators were highly significant level. A total of 16 copies of materials phenols and flavonoids, DPPH and ABTS values ??cluster analysis, according to the Euclidean distance is more than 16 parts of material 70 may be divided into two categories. Which contains 11 parts of class Ⅰ materials (including Emei Houttuynia), its low content of polyphenols and flavonoids, antioxidant capacity is weak. Ⅱ class contains five copies of material, its high content of polyphenols and flavonoids, antioxidant ability. This five materials were W01-52, W01-99, W01-94, W01-98 and W01-100.

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