Dissertation > Excellent graduate degree dissertation topics show

Expression and Clinical Significance of Asporin Ptotein in Human Degeneration Discs

Author: LiangGuoFeng
Tutor: ZhuYue
School: China Medical University
Course: Surgery
Keywords: Asporin Collagen type II Gathered proteoglycan Prominent Prolapse
CLC: R681.53
Type: Master's thesis
Year: 2009
Downloads: 59
Quote: 0
Read: Download Dissertation

Abstract

The purpose of lumbar disc degeneration (lumbar disc degeneration, LDD) is the main cause back pain. About 80% of people in their lifetime have experienced back pain, low back pain to bring great pain, high medical costs, as well as the significant impact of the social economy, especially the loss of work time in the world to serious medical and economic burden to society. Disc degeneration (intervertebral disc degene ration, Ⅳ DD) is a the fundamental pathological basis of a range of degenerative diseases of the spine, the outcome is often spinal stenosis, spinal segmental instability, osteophyte formation, Yao Tuiteng, cervical spondylosis, disc and consequent nerve root, spinal cord compression, the clinical onset complex disease. Lumbar disc herniation, the most representative. Therefore, it is particularly important to the study of the mechanism of lumbar disc herniation and degenerative. The Asporin protein was found in 2001 in the articular cartilage and meniscus, according to the characteristics of its molecular structure is divided into a new member of the family of small leucine-rich proteoglycan (SLRP), a member of the family of proteins according to the amino acid sequence characteristics and genetic makeup are divided into four groups of the Asporin protein and Decoin protein and protein Biglycan together are classified in the first group. The study found that the protein is abundantly expressed in the articular cartilage of osteoarthritis, it is considered that its closely related and osteoarthritis. OA and LDD are degenerative bone and joint disease, the expression of many genes in the articular cartilage in the intervertebral disc has the same expression, so we speculate ASPN should be a susceptibility gene for LDD. Therefore, this study was designed to study asporin protein expression in herniated lumbar disc. Materials and Methods 1, samples should be taken from Chinese Medical University First Clinical Hospital orthopedic March 2008 to November 2008 lumbar disc herniation surgery to remove the nucleus pulposus specimens of 25 patients, aged 26-76 years old, including 12 men patients, 13 females, divided according to the classification criteria of the International the lumbar Research Institute of prominent and prolapse, which highlights 12 cases (prominent group), 13 cases of prolapse (prolapse group). Specimens after removing the nucleus pulposus and annulus tissue separation, take the nucleus pulposus stored at -70 ℃. Control group of five cases taken from the lumbar spine fractures in patients with anterior surgery for removal of the intervertebral disc, aged 21-45 years old, 3 males and 2 females, degenerative disc had no signs of the annulus fibrosus complete, the annulus fibrosus and nucleus pulposus boundaries clear (control group). 2, Western blot (western blot) the method detection asporin protein expression in 25 cases of degeneration of the intervertebral disc nucleus pulposus, 5 cases of normal intervertebral disc tissue. 3 Results found computer imaging gray value determination by comparing the sample protein and within the reference optical density of β-actin, and the results of semi-quantitative analysis of protein and tissue samples (forthcoming organization constant expression of β-actin protein gray value for comparison), and eventually come to the target protein in the relative content of degeneration between the disk and the normal intervertebral disc tissue. 4 All data were statistically SPSS13.0 software for statistical processing of measurement data using analysis of variance, P <0.05 considered statistically significant. 1, the normal intervertebral disc tissue and degeneration of the intervertebral disc type II collagen expression of type II collagen expression in normal intervertebral disc tissue were 1.6 times 1.4 times prominent group and prolapse group, there were significant differences (P <0.01); prominent group expression of collagen type II is a the prolapse group of 1.1 times, there was no significant difference (P = 0.07). Normal intervertebral disc degeneration intervertebral disc tissue expression of aggrecan gathered proteoglycan expression in normal disc tissue is a prominent group of 1.0 times, there is no significant difference (P = 0.06); is prolapse group 1.5 times, there is a significant difference (P <0.01); expression aggregation proteoglycan projecting group Escape 1.4 times, there are significant differences (P <0.01). Normal between the disc and degenerative intervertebral disc tissue asporin protein expression Expression of Asporin protein prolapse group is a prominent group of 1.2-fold, without significantly with difference (P = 0.06); is 1.5 times that of the normal group there are significant with sex The difference (P <0.01); projecting group is 1.3 times that of the normal group, there was significant difference (P <0.05). Conclusion 1, Asporin protein intraday expression between normal and prominent people. Expression intraday Asporin protein prominent between increased significantly.

Related Dissertations

CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Bone diseases > Spine and back disorders > Spinal joint disease
© 2012 www.DissertationTopic.Net Mobile