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Experimental study in rats with acute spinal cord injury after the activation of autophagy (autophagy) changes in the time course of expression to explore autophagic cell behavior in acute spinal cord injury , and to provide a new strategy for the treatment of spinal cord injury and the new target . The experimental method healthy Wistar rats , male, weighing 250 ± 20g, were randomly divided into normal control group and injury group for transmission electron microscopy detection take 20 Another 50 were randomly divided into normal control group and injury 2h group , 6h , 24h , 48h group for Western blot analysis . Rats using a modified Allen's Preparation of rat model of acute spinal cord injury , the the TEM observations normal control group and the injured group corresponding to the area of spinal cord tissue ultrastructure detect autophagy (autophagosome) formation , detected by Western blotting normal control group and injury 2h , 6h , 24h , 48h group of the corresponding region of spinal cord tissue autophagy -related protein LC3 ( microtubule -associated protein 1 light chain 3 ( microtubule associated protein 1 light chain 3 , MAP1 - LC3) ) expression . Experimental results of transmission electron microscopy , the rats of normal control group and the injury group ultrastructure image display : the normal control group rat spinal cord neurons nuclear membrane integrity , the karyotype rules , the cytoplasmic mitochondrial morphology distribution and the number of normal , and no self- macrophages bodies , lysosomes number no increased ; damage zone of injury group of neurons , the nucleus shape irregular , rough endoplasmic reticulum , lysosomes increase in the number of synapses and axons of the neurons are visible autophagic bodies , and visible swelling of mitochondria ; Western blot results show : the normal control group LC3 Ⅱ / LC3 Ⅰ the ratio lower SCI after 2hLC3 Ⅱ / LC3 Ⅰ the ratio compared with the control group that have significantly increase ( P < 0.05 ) , SCI after 6h LC3 II / LC3 Ⅰ the ratio peak (P <0.01), and continued to 48h is still higher expression . Autophagy is activated conclusions after acute spinal cord injury in rats , and its expression has some schedule changes , activation lasted at least 48h autophagy may played an essential role in spinal cord injury .
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