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Objective To observe the Mai-Ling Qi Gui benefits of acute myocardial ischemia-reperfusion myocardial apoptosis related genes Fas , Bcl -2 protein expression , investigate the myocardial protective effect and its mechanism . Methods 70 Wistar rats were randomly divided into seven groups ( n = 10 ) and No. : blank control group , sham operation group , ischemia-reperfusion group , ischemic preconditioning group, isosorbide dinitrate group Mai-Ling Qi Gui benefit low-dose group and Mai-Ling Qi Gui benefits the high- dose group , the left coronary artery the threading ligation prepared myocardial ischemia -reperfusion model , taken after the apex organization successful modeling light microscopy with HE staining morphological changes of apoptotic cells were observed under ; immunohistochemical staining for detection of myocardial cell apoptosis related genes Fas , Bcl -2 protein expression situation . Results 1 . Except the sham group and the control group , and the remaining group of myocardial cells have different degrees of morphological changes to ischemia -reperfusion group cell morphology injury with . Qi Gui benefits veins spiritual high , the low- dose group , isosorbide dinitrate group , ischemic preconditioning group and ischemia-reperfusion group increased the expression of Bcl-2 protein , a significant difference ( P <0.05 ) ; Mai-Ling Qi Gui benefits high dose group significantly increased Bcl-2 protein expression was significantly (P <0.05), compared with isosorbide dinitrate group compared with the low- dose group differences , no significant difference ( P > 0.05 ) between the two . 3 . Ischemia re- perfusion group Fas protein expression in the highest , and the remaining groups , a significant difference ( P < 0.05 ) Qi Gui benefits veins spiritual group can significantly lowered expression of Fas protein , and high-dose group significantly with , its role and isosorbide dinitrate group , ischemic preconditioning group fairly among difference was not significant ( P > 0.05 ) . Conclusion 1 . Mai-Ling Qi Gui benefits reduce acute myocardial ischemia and reperfusion in the rat model of myocardial structure abnormalities . Mai-Ling Qi Gui benefits through of Fas gene expression of Bcl -2 gene protein expression inhibition of cardiomyocyte apoptosis , certain anti- myocardial injury . Qi Gui benefits Mai-Ling regulation of apoptosis gene and ischemic preconditioning group , isosorbide dinitrate group fairly , suggesting that it has a cardioprotective effect of pharmacological preconditioning .
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