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Objective: The main purpose of this study: 1, try to use \2, to study the material basis and mechanism of action of the treatment of stomach soup FD prescription welcomes explore the scientific basis of its overall modulating FD. Method: Step 1: 8-week-old male Wistar rats modeling and treatment SPF level of 40, body weight (200 ± 20) g, were randomly divided into four groups, each group of 10. Take a group as the control group (BG), normal feeding for 14 days. The remaining three groups according to the irregular feeding with the tail clamp stimulation modeling for 14 days. Blank group with saline gavage for 14 days; remaining three groups were known as the model control group (MG), the treatment group (ZG), domperidone treatment group (DG), respectively, with saline welcomes stomach soup decoction, domperidone solution gavage for 14 days. Observed before and after treatment the rats in each group general condition, body weight and food intake. Step 2: Indicators gastric emptying experiments: the 4 groups of rats fasted water for 24 hours, with starch paste (1g/ml) 10mg/kg orally, sacrificed by decapitation, after standing for 1 hour blood 5ml first into the anticoagulant tube refrigerated spare analyte NO, MTL; cut open the abdominal cavity, stomachs, weighed, cut open the stomach wall, wash away the contents, and then said the empty stomach weight calculation gastric emptying rate and observed gastric mucosa. Cut the antrum into a 10% solution of formaldehyde fixed, the rest of the stomach tissue developed into 10% homogenate spare analyte NO, nitrate reductase assay stomach wall tissue and serum NO content; using RIA assay plasma MTL content. The antrum organizational structure observation: the emptying experiment specimens from the antrum specimens removed graded ethanol dehydration, xylene, paraffin-embedded, 4μm sections, HE staining in antrum organizational structure was observed under an optical microscope. Results: 1, the general status and gastric mucosal morphology blank steady increase in food intake rats; significant weight gain; coat color is white, soft and smooth; stool forming little odor; milder temperament, good spirit; gastric reddish surface . The model rats eating disorders, increased water intake; slow weight gain; coat color yellow dry; feces shapeless foul smell unpleasant; irascible and mental exhaustion; gastric mucosal congestion and edema, surface integrity. Domperidone group and Chinese medicine group before treatment is basically the same with the model group, after the therapeutic food intake, weight, hair color, feces were improved. Than before treatment mild temperament, the spirit of improvement. The gastric mucosa reddish surface integrity. 2, gastric emptying rate (%) with the model group (16.22 ± 3.62) compared welcomes stomach soup group (28.19 ± 1.65) was significantly higher in the gastric emptying rate (P <0.05), and the Chinese medicine group was significantly superior to domperidone group (22.69 ± 0.96) (P <0.05). 3, stomach tissue and serum NO content in stomach tissue NO content (μmol / gprot): model group (4.18 ± 0.12) comparison, traditional Chinese medicine group (2.80 ± 0.23) can significantly with lower gastric tissue NO content (P <0.05), and significantly better than domperidone (3.76 ± 0.21) (P <0.01). Serum concentration of NO (μmol / L): with the model group (59.75 ± 10.86) comparison, traditional Chinese medicine group (42.46 ± 4.54) with a significant difference (P <0.05); Chinese medicine group and western medicine group (47.51 ± 9.59) compared to no significant differences (P> 0.05). 4, plasma MTL content (pg / ml) and model group (385.12 ± 10.89), traditional Chinese medicine group (430.03 ± 8.23) there are significant with differences (P <0.01); western medicine group (408.28 ± 11.10) relative to comparison there is a significant difference ( P <0.05). Antral organizational structure blank rats antral organizational structure is basically normal mucosa integrity, no congestion, edema and inflammatory cell infiltration, the shape of the rules of the gastric glands. The model rats antral mucosa, submucosal telangiectasia the gastric glands gap wide and visible obvious inflammatory cell infiltration. The integrity of the the antral mucosal layer of the western medicine group and Chinese medicine group, no significant congestion, edema and inflammatory cell infiltration, the shape of the rules of the gastric glands. Conclusion: 1, Yan stomach soup has overall modulating effect on the FD model rats, mainly through the following mechanisms: to improve the the FD rats General status and gastric mucosal morphology; improve solid gastric emptying rate in rats; reduce FD rat stomach tissues and serum NO content, thus reducing NO inhibition of gastric emptying; enhance the the plasma MTL content of FD rat plasma, thus promoting gastric emptying. 2, the present study demonstrated that Chinese medicine Xin stomach soup both directly promote gastrointestinal motility (increase gastric emptying rate), but also to regulate the level of gastrointestinal hormone; combined with the party's past studies have shown that the functionality associated with depression and anxiety state dyspepsia have significant effects; therefore welcomes the stomach soup combines Western medicine to promote gastrointestinal motility drugs, antidepressants and regulate gastrointestinal hormone characteristics, embody the characteristics of the overall concept of Chinese medicine treatment of diseases.
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