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Purpose doctrine to Meridian meridians (points) - the theory organs as a guide to select different acupuncture point (Neiguan Lieque, too deep, Algol, Zusanli beam the mound, Yanglingquan, Wind City) ventricular arrhythmias electro-acupuncture treatment in rats nerves on the electrocardiogram, cardiac sympathetic and Xinmi discharge the heart autonomic ganglia detection of acetylcholinesterase (Acetylcholines-terase, AChE), different acupoints electricity for normal and ventricular arrhythmias in rats ECG, cardiac sympathetic and vagal nerve activity, and to explore the differences and mechanisms of Acupuncture effect. Methods SD rats were 200, weight 200 ~ 250g, male, were randomly divided into normal group (A, 10), model group (group B, 10), a simple electro-acupuncture group (C, 80), electro-acupuncture treatment group (D, 80). Group C and Group D is divided into C1-C8 subgroup and D1-D8 subgroup within the customs Lieque too deep, Algol, Zusanli the beam the mound, Yanglingquan, Wind City eight subgroups, Each subgroup rat 10. The cesium chloride intravenously induced ventricular arrhythmias modeling various acupuncture points, respectively, EA 5min needle retention 10min electrophysiological methods to record the rat ECG, cardiac sympathetic and vagal discharge; use of histochemical assay sympathetic, parasympathetic center of AChE activity. Results 1. ECG: ECG modeling EA performance typical of ventricular contraction, heart rate slowed down, with a significant difference (P <0.05); simple EA group before and after electro-acupuncture was no significant difference; treatment group, subgroup EA, ECG Room premature contraction performance disappear, heart rate than EA have different levels of recovery, with a significant difference (P <0.05) between the subgroups compare the Neiguan treatment group heart rate increased most In obvious, Algol, Zusanli second, compared with other subgroups with significant differences (P <0.05). 2. Nerve discharge: modeling fans walk nerve firing decreased sympathetic discharge increased, both with a significant difference (P <0.05); pure electric needle group each subgroup electric needle before and after comparison, vagus, sympathetic discharge had no significant forward difference; with the electric needle before each subgroup electric needle electro-acupuncture group comparison vagus nerve discharge increased in varying degrees, sympathetic discharge reduction in varying degrees, all have significant differences in sex (P <0.05); subgroup group comparison show electro-acupuncture The Neiguan strongest effect on autonomic regulation, Algol second compared with other subgroups with significant differences (P <0.05). 3.AChE gray values: normal group in the medulla oblongata vagus nerve nucleus and spinal lateral angle AchE positive nuclei were not colored, the cytoplasmic brown yellow, gray values ??were 70.29 ± 7.91,71.48 ± 6.20; modeling AChE gray value greater than the normal group, with significant differences (P <0.05); the simple EA group subgroup AChE gray value compared with normal group without significant differences; subgroup gray value of the electro-acupuncture group were less than making modules, and has a significant difference (P <0.05), the subgroup of group comparison show the Neiguan acupoint AChE grayscale value is less than the other sub-group, and has significant differences (P <0.05). Conclusion 1. Electric Neiguan, the Daling 8 acupoints can improve ventricular arrhythmias in rats of varying degrees of cardiac electrical activity, but different acupoints acupuncture effect differences, Neiguan needle efficiency most obvious Great Mausoleum second. Electroacupuncture of Different Acupoints able the heart autonomic varying degrees of adjustment arrhythmia (sympathetic and parasympathetic) excited state, the strongest the Neiguan regulation effect. Electrical differences in cardiac autonomic function may be one of the important reasons for its anti-ventricular arrhythmias acupoint needle efficiency differences. 3. The different acupoints electric needle could affect central neuronal AChE activity Neiguan EA treatment group showed the highest activity. Electricity for the the autonomic central neurons function of differences may be one of the reasons for its anti-ventricular arrhythmias acupoint needle efficiency differences, and possibly through the regulation of sympathetic and fans nerves activities exert its effect.
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