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Screen of High-lipid Content Marine Microalgae Strains Based on Preparation of Biodiesel

Author: YuYang
Tutor: GaoYaHui
School: Xiamen University
Course: Hydrobiology
Keywords: Microalgae Filter Lipids Mutagenic Biodiesel
CLC: TE667
Type: Master's thesis
Year: 2009
Downloads: 1145
Quote: 7
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Abstract


The development and utilization of bio-energy is an important way to ease the pressure on global energy and energy security. Biodiesel for its high level of sustainability and clean pollution-free characteristics become the focus of global attention. Microalgae have a high fat content, large amount of biological growth cycle is short, culture is a good resource for biodiesel production. The purpose of this study is to be obtained by filtering algae species and Mutation Breeding of high lipid content and fast growth of microalgae, and optimization of culture conditions and training methods, to provide a theoretical basis and technical support for large-scale production of microalgae biodiesel. The main findings are as follows: 1) study showed that four benthic diatoms collected on a laboratory the existing algae species and wild Determination of total lipid screening as fat microalgae: Phaeodactylum tricornutum (Pheaodactylum tricornutum ), the total lipid content of 34.4%; Chaetoceros muelleri (Chaetoceros muelleri), total lipid content of 25.1%; spin chain Chaetoceros (Chaetoceros curvisetus), 24.2% of the total lipid content; fake miniature Thalassiosira pseudonana (Thalassiosirapseudonana), total lipid content of 24.3%; halophyte Navicula (Navicula halophila), 39.1% of the total lipid content; the debris nitzschia symbiotic the variants (of Nitzschia frustulum var.symbiotica), 35.7% of the total lipid content. Sludge of two benthic diatoms, the halophytic Navicula debris nitzschia symbiotic variant of total fat content is higher. 2) Nile Red dye, false micro Thalassiosira pseudonana staining and fluorescence intensity was measured by comparing the standard substance derived fat content. Optimized dyeing conditions Nile Red concentration 0.5μg/mL, dyeing time 10min, dyeing temperature 40 ° C the algae solution OD 680 the range of 0.1 to 0.4. The results show that the optimization can be accurately determined the fake miniature Thalassiosira pseudonana and Chaetoceros muelleri neutral lipid content Phaeodactylum algae ineffective. 3) using ultraviolet irradiation of a fake miniature Thalassiosira algae mutagenic treatment, weighing Nile Red fluorescence and grease extraction method to complete the screening and rescreening. 7 mutant in HL-1 mutant total lipid content than the original strain original content of 19.3%, the growth rate is lower than the original strain, but biomass is higher than the original strain. 4) On this basis, through orthogonal experiment and found that the optimum growth conditions of the two microalgae and optimum oil-producing conditions. The Phaeodactylum tricornutum optimum growth and production of Grease conditions were light 4500lx, temperature 17 ° C, NO 3 - concentration 2mmol / L, EDTA-Fe the concentration of 10 -4 mmol / L as light 3000lx, temperature 22 ℃, NO 3 - concentration of 0.5mmol / L, EDTA-Fe concentration 10 -6 mmol / L. With Chaetoceros muelleri optimum growth and optimum production Grease conditions were light 6000lx, and a temperature of 27 ° C, NO 3 - concentration 2mmol / L, EDTA-Fe concentration 10 -6 mmol / L and light 3000lx of temperature 17 ° C, the NO 3 - concentration of 1mmol / L, EDTA-Fe concentration 10 -5 mmol / L. 5) as a raw material to the oil extracted from a variety of fat microalgae preparation of biodiesel, the yield was 5.6 to 15.3% of the dry weight. Which the halophyte NAVICULA and debris nitzschia symbiotic variants biodiesel production is higher than the other three fat diatoms, 15.3% and 14.3%, respectively.

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CLC: > Industrial Technology > Oil and gas industry > Oil, natural gas processing industry > Synthetic oil > Extraction of oil from other raw materials
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