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In this study include three parts, the first part of the Dragon and carp (♂) × the koi (♀), Dragon and carp (♂) × Longfeng carp (♀) for the parent to obtain two sets of offspring Dragon and carp (93), measuring the two Dragon and Phoenix the carp's 10 main morphological traits and 20 frame structure parameter group of offspring, parents studied offspring form; albacore carp, koi and dragon and phoenix carp of 76 individuals as part of the research material from carp microsatellite primers screened 20 Dui cited material, the use of microsatellite molecular marker methods, analysis of genomic DNA in 20 micro-satellite-bit point genetic differences; third part of a long fin carp 8 Wei Koi 8 Wei and Longfeng carp individuals 10 tail as the research object, using mitochondrial DNA D-loop region of molecular markers to study the genetic differences and genetic relationship of the three kinds of carp in the non-coding region of the mitochondrial DNA, the main findings are as follows: 1, two groups of offspring 30 morphological indicators through mean t-test, principal component analysis, discriminant analysis and the difference in coefficient test method analysis, two groups of offspring only in body thickness / width, the pectoral base starting point to head dorsal end of BC / body, the starting point for the base of dorsal fin to anal fin base starting point for EF / body length and dorsal fin base starting point to the end of the anal fin base four indicators EH / body length with a significant difference (P lt; 0.05), and the remaining indicators were no significant differences; nine principal component principal component analysis, the cumulative contribution rate 81.11%, principal component analysis diagram shows no significant deviation from the trend between each other; discriminant analysis to get the body thickness / length, snout length / body length, the eye spacing / length, pectoral fin base starting point to pelvic fin base starting point for BD / length pectoral fin base starting point to head dorsal end of BC / body length and pelvic fin base starting point to head the dorsal end of the DC / 6 indicators discriminant function analysis of factors, the two groups of offspring discrimination accuracy was 78.5%; The difference in coefficient test showed the 30 morphological indicators of differences in coefficient values ??were less than 1.28, there is no division of the sub-species standard. Overall judgment by Dragon and carp (♂) × offspring the Longfeng carp (♀) and has a high degree of similarity in morphology by the Longfeng carp (♂) × Koi (♀) offspring. 2, using 20 microsatellite primer albacore carp, koi and the Longfeng carp genetic diversity study, three groups were found in 20 sites to 108,164,154 alleles were detected, effective etc. number of alleles were 4.496,5.695 and 5.506, genetic diversity were 0.764,0.803 and 0.799, expected heterozygosity were 0.769,0.806 and 0.801, polymorphism information content (PIC) were 0.703,0.761 and 0.757, the overall genetic higher levels of diversity, the Koi highest genetic diversity, followed by Dragon and carp, albacore carp minimum. Hardy-Weinberg equilibrium test showed that the three groups, a total of 26 loci performance of the genetic imbalance highly significant (P lt; 0.01), deviation index analysis showed: three groups of 20 microsatellite loci in Miscellaneous state zygote surplus. The average genetic differentiation coefficient of 7.1%, and the average of the gene flow (Nm) 3.5895,3 populations greater gene flow between. Albacore carp, koi and dragon and phoenix carp mitochondrial DNA D-loop region sequences analysis of genetic structure and relationship length analysis of 386 bp fragment, the fragment is rich in AT GC content less were found 17 variable sites, accounting for 4.66% of total sites, seven single polymorphic sites, 10 parsimony informative sites, observed a base deletion at 174 bp A insertion. Found a total of 10 kinds of haplotypes which two kinds of haplotype sharing for groups, others are groups specific population mean haplotype variation of 0.708 ± 0.095,3 groups average nucleotide variation and nucleotide polymorphisms were 3.966 and 0.0103, Genetic cluster analysis shows Longfeng carp and koi genetic distance, far albacore carp genetic distance, Dragon and carp in the three groups in many genetic traits with Kam Similar carp, koi genetic diversity indicators than albacore carp and dragon and phoenix carp, the results displayed microsatellite analysis.
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