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Analysis of Genetic Diversity and Origin of Chinese Game Chickens

Author: XueSuMei
Tutor: LiAng；ChenKuanWei
School: Fujian Agriculture and Forestry University
Course: Animal Genetic Breeding and Reproduction
Keywords: China cockfighting Microsatellite Mitochondrial DNA cytochrome b Genetic Diversity Phylogenetic tree
CLC: S865
Type: Master's thesis
Year: 2009
Downloads: 70
Quote: 0
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Abstract


Poultry genetic resources in China is very rich, where cockfighting as a phenotype very special breed, training history, background and role is not the same with the other chicken breeds. China's major five cockfighting varieties are Turpan (Xinjiang), Xishuangbanna (Yunnan), the Luxi (Shandong), Henan Game (Henan) and Zhangzhou cockfighting (Fujian). Polymorphism of microsatellite loci and mitochondrial DNA cytochrome b gene genetic polymorphism of the 5 the cockfighting varieties of this study in the application of microsatellite markers and mtDNA the cytb gene molecular genetic markers, the use of direct DNA sequencing method The detection and analysis of genetic distance between the various the cockfighting varieties of genetic diversity, and discussed the origins of cockfighting in China. 1 using 27 microsatellite loci of the cockfighting China system clustering analysis, statistics of the effective number of alleles, the genetic distance between the polymorphic information content, heterozygosity and varieties. The results show that cockfighting groups in Henan heterozygosity low as 0.532, Henan Game genotypes consistent paucity of population genetic diversity; the Xishuangbanna groups heterozygosity highest was 0.662, with a wealth of genetic diversity; Luxi Xishuangbanna genetic distance, 0.206; the farthest the Zhangzhou cockfight and Ye Lu Fan cockfighting genetic distance, 0.516. The clustering results show that the the Zhangzhou cockfighting and the Central Plains cockfighting genetic distance, alone in a class, Xishuangbanna and Turpan are descendants of Luxi, among the short distance, clustered into a large class of that, Zhangzhou The cockfighting foreign cockfighting descent differences with China cockfighting origin. 2 using mitochondrial DNA cytochrome b gene measured 5 cockfighting varieties 40 individual mitochondrial DNA part of the base sequence of the cytochrome b (535bp), the study found a total of six haplotypes, haplotype diversity 0.42857-0.78571; nucleotide diversity 0.00081-0.00659, Zhangzhou cockfighting genetic diversity of the most abundant and the the Xishuangbanna and Turpan most poor. Zhangzhou cockfighting cockfighting genetic distance farther, and analytical methods used microsatellite markers were consistent. 40 cockfighting sequences and GeneBank red jungle fowl corresponding sequence comparison and phylogenetic tree built haplotype-based system, the results show that China the cockfighting The matriarchal may be derived from the red jungle fowl subspecies.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Hunting,domestication of wild animals > A variety of wild animal domestication
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