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Noise-induced Alteration in Expression Patterns of Cystathionine-γ-lyase in Rat Cochleae

Author: MaoXiaoBo
Tutor: QiuJianHua
School: Fourth Military Medical University
Course: Otorhinolaryngology
Keywords: Cystathionine -γ- lyase Rats Cochlea Noise exposure
CLC: R764
Type: Master's thesis
Year: 2009
Downloads: 35
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Abstract

Purpose With the continuous improvement of the level of urbanization and industrialization , the noise on the health hazards is increasing day by day , and long-term exposure to noise can have adverse effects on many systems of the human body , especially in the auditory organ damage is the most prominent . Numerous studies show that the inner ear microcirculation, hearing loss caused by noise . Therefore , the research on cochlear microcirculation has been the focus of attention of otology sector . In recent years , hydrogen sulfide (H2S) gas signaling molecules to improve the the vascular microcirculation of important research direction . Cochlear vascular microcirculation theory based on the endogenous H2S physiological characteristics and vascular effects , as well as the process of noise-induced hearing loss , from H2S start in the rat cochlea distribution and regulation function , observed cystathionine -γ- lyase (cystathionine-γ-lyase, CSE) in the rat cochlea distribution and expression changes in noise stimulation , in order to further explore the role of hydrogen sulfide in the inner ear microcirculation in the prevention of noise-induced hearing loss , experimental ideas and basis open new avenues for the prevention and treatment of the disease of the inner ear and the microcirculation . By immunohistochemistry and immunofluorescence staining techniques to observe the distribution and positioning of the CSE in normal adult SD rat cochlea . Noise exposure to 32 SD rats were randomly divided into four groups : normal control group one day , one week , three weeks after the 3 groups were exposed to white noise of 110 dB SPL broadband . By reverse transcription polymerase chain reaction (RT-PCR) was detected in rats receiving strong noise before and after stimulation CSE mRNA expression changes within the cochlea after noise exposure . Results of immunohistochemistry and immunofluorescence CSE expression in the vascular pattern of the rat cochlea and cochlear tiny blood vessels at the membrane ; cochlear inner and outer hair cells , various types of support cells , at the cochlear ganglion showed no expression . RT-PCR results display the noise stimulation time , CSE mRNA level was the first growth after a downward trend . Conclusion CSE clear expression in the cochlea , before and after noise exposure may be involved in the regulation of cochlear microcirculation , suggesting that may play an important role in improving cochlear blood circulation . The above study the pathogenesis of noise-induced hearing loss , prevention and treatment to provide a new way of thinking .

Noise-induced Alteration in Expression Patterns of Cystathionine-γ-lyase in Rat Cochleae

Abstract

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