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Cerebral infarction is a common disease caused by neurological deficit, actively and effectively promote cerebral infarction ischemic recovery period recovery of neurological function, is still cerebral infarction focus of the study. The discovery of neural stem cells to break the view can not be repaired in the adult central nervous system injury, and also provides a new approach for the treatment of cerebral vascular diseases. A large number of studies have demonstrated that cerebral infarction can activate the brain of adult-derived neural stem cells, induced neurogenesis. Regeneration of nerve tissue after cerebral infarction, including neural stem cell proliferation, migration and differentiation of three basic process. Damage zone of endogenous neural stem cell proliferation and migration, differentiation, generate new nerve cells to be partially restored. However, the small number of endogenous neural stem cells and neurogenesis by various factors, therefore, how to activate endogenous neural stem cells and to promote their differentiation into neurons has become the focus of current research. One of the difficulties is the use of neural stem cells in the treatment of cerebral vascular diseases. The hippocampus is the key parts of learning and memory, learning and memory dysfunction hippocampal damage caused by persistent and difficult to restore. The hippocampus is one of the regions of adult neurogenesis, the selection of the photochemical method rat hippocampus infarction model, the proliferation and differentiation of neural stem cells in the area studied in order to observe the water maze training. Water maze training is a rehabilitation method, in previous studies, we have found that water maze training can increase the expression of nerve growth factor to promote the migration of neural stem cells, and enhance self-repair after brain injury, to improve the impaired learning memory function. Water maze training on the proliferation and differentiation of neural stem cells after cerebral infarction is rarely reported. By the photochemical method, the experimental rat hippocampal infarction model given water maze training, observe the water maze training on hippocampal infarction rat neural stem cell proliferation and differentiation, and explore water maze training and central nervous system plasticity for clinical cerebral vascular rehabilitation of patients with diseases provide a theoretical basis. The experiment is divided into two parts: the first part of the water maze training Objective To explore the dentate gyrus of BrdU and Nestin expression in rat hippocampal infarction the water maze Training proliferation in the dentate gyrus of rat hippocampal infarction neural stem cells. Methods photochemical into the legal system of unilateral hippocampal infarction model in rats 72 were randomly divided into the the infarction training group (n = 36) and pure infarction group (n = 36), each group set 1,7,14,21,28 and 35d of six subgroups. And normal control group of 36, corresponding to the model group is divided into 1,7,14,21,28 and 35d of six subgroups. The infarction training rats given water maze training in modeling 1d, simply infarction rats freedom of movement, not water maze training. Double-labeling immunofluorescence was used to observe the different time points dentate gyrus bromodeoxyuridine double-labeled uracil nucleoside (Bromodeoxyuridine BrdU) and nestin (Nestin) expression. Results normal control group dentate gyrus area has a small amount of BrdU / Nestin double labeling positive cells infarct training group and simply infarction rats at 14, 21 and 28d hippocampal infarction side dentate gyrus area BrdU / Nestin double-labeled The number of positive cells were significantly increased (P lt; 0.01); the infarction training rats 7,14,21,28 d double labeling the the infarction side of the dentate gyrus of the BrdU / Nestin positive cells was significantly higher than infarction ( P lt; 0.01); to 35d infarction training group and simply infarction rat hippocampus double labeled infarction side of the dentate gyrus area BrdU / Nestin positive cells with normal control group no significant difference (P GT; 0.05). Conclusion water maze training can significantly enhance the infarction of rat hippocampus dentate gyrus of neural stem cell proliferation, and promote recovery of neurological function. The second part of the water maze training infarction of rat hippocampus dentate gyrus of neural stem cell differentiation capacity Objective To study the water maze training dentate gyrus of rat hippocampal infarction ability of neural stem cells. 162 SD rats were randomly divided into the infarction training group, simple infarction group and the normal control group. Each group set 1,7,14,21,28,35,42,49 and 60d of nine subgroups. Photochemical method infarction training group, simply infarction rats made unilateral hippocampus infarction model, the infarction training rats given water maze training the next day after modeling, simple infarction rats after modeling, freedom of movement, not to water maze Training. Immunofluorescence double staining observed rats in each group at different time points dentate gyrus bromodeoxyuridine (Bromodeoxyuridine BrdU) and neuronal core antigen (Neuronal nuclei antigen, NeuN) and glial fibrillary acidic protein (Glia fibrillary acidic protein, GFAP) double labeled. The normal control group dentate gyrus and a small amount of BrdU / NeuN and BrdU / GFAP positive double labeling staining; Compared with normal control group, the the infarction training group and simply infarction group dentate gyrus BrdU / NeuN and BrdU / BrdU double labeling staining of GFAP positive 14,21,28,35,42,49 days after surgery has increased (P lt; 0.01); infarction training rats after 14,21,28,35,42,49 d / NeuN and BrdU / GFAP positive double labeling staining were significantly higher than the MI group (P lt; 0.01) To 60d, infarct training group and simply infarction group dentate gyrus area BrdU / NeuN and BrdU / GFAP positive double labeling staining with the normal control group were not significantly different (P gt; 0.05) Conclusion water maze training can significantly enhance the ability of hippocampal neural stem cells differentiate into neurons and astrocytes, has an important role to promote the recovery of neurological function.
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