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The herbal compound Gegenqinlian from \The traditional Chinese herbal compound study pharmacokinetic study of single herb and the compound after the administration of one or more active ingredients, but this does not reflect the the Compound overall body dynamic process. Based on the substance of the serum containing the ingredient is direct in vivo role of traditional Chinese medicine, the serum drug chemistry research ideas to the oral Gegenqinlian rats plasma as the research object, the integrated application of modern technology, from serum isolated and identified transitional ingredients, to study its correlation with traditional efficacy and build plasma pharmacokinetics fingerprints, to clarify the direct effect of in vivo metabolism of substances and the dynamic process of the body. Specific content is divided into the following four parts: (1) as the research object, the establishment of Gegenqinlian to the rat oral Gegenqinlian plasma decoction into the blood component analysis method: interference analysis of biological samples measured component protein . This study screened out by experiment methanol precipitation of plasma protein in the pretreatment method, the samples were dried with nitrogen, with methanol after reconstitution to 12000 r / min, centrifuged after sample obtained directly used for HPLC analysis. By optimizing the wavelength and the pH of the mobile phase HPLC conditions, it is determined at a wavelength of 270 nm, the rat plasma samples by gradient elution with acetonitrile and 20 mM ammonium acetate solution. 2 attribution Gegenqinlian into the blood component: Compare blank plasma Jiage of root Scutellaria and Coptis extract, the rats were orally administered compound and the single-agent drug-containing plasma chromatogram, after administration of the compound into the blood component vesting . Learn from research ideas of the fingerprint, retention time, the attribution of the 31 ingredients in the compound containing drug plasma, including 15 from Chinese medicine, 13 metabolites, three new substances into the body . Established liquid chromatography - diode array - mass spectrometry (HPLC-DAD-MS) analysis of plasma samples the rat oral Gegenqinlian after identification method: First puerarin, baicalin, berberine to optimize MS conditions palmatine and jatrorrhizine five standard injection analysis mass spectrometry positive and negative ion mode, respectively, according to the charge to mass ratio of retention time, UV wavelength of maximum absorption and the positive and negative ion mode, with the standard or References preliminary appraisal or inferred the rat oral Gegenqinlian plasma of 14 prototype pharmaceutical ingredients, were puerarin, 3'-methoxy puerarin ,8-C-celery glycosylation ( 1 → 6) glucose - daidzein, daidzein, baicalin, wogonoside celery sugar base-7-O-β-D-glucose anhydride acid, wood butterflies prime A-7-O-β-D- glucuronide jatrorrhizine coptisine, berberine, palmatine, glycyrrhizin, glycyrrhizin. Flavonoids in the body susceptible to the first-pass effect, glucuronidation is the major metabolic pathway in the plasma of this study speculated that the four kinds of metabolites, respectively, for the daidzein-7-O-of β-the D-glucoside acid, soybean aglycone 4'-O-sulfate ester, baicalein-6-O-β-grape pyran uronic acid glycosides ,6-O-methyl of - scutellarein-7-O-sulfate ester, and identification of these components will help on elucidating the potential basis Gegenqinlian. 4 Constructing the plasma pharmacokinetics fingerprint, and the first to chemometrics for Gegenqinlian into blood components by changing process: First the chromatographic methods of drug-containing plasma methodological study constructed plasma pharmacokinetics fingerprinting results show good stability, high reproducibility, and suitable for the pharmacokinetic study. Subsequently investigated the decoction after oral Gegenqinlian in rats within 48 h 12 major ingredient in the process of dynamic changes in the body. Rapid metabolism of Pueraria main ingredient puerarin peak time of 1 h and 10 h after administration of basic metabolic finished; licorice, some of the compounds of the peak time of 1-1.5 h; skullcap in the main component baicalin bimodal phenomenon, the peak time was 2,10 h, some compounds similar to its structure and baicalin showed irregular multimodal phenomenon with enterohepatic circulation of the body. Different time of blood sampling point chromatography data import SIMCA-P software principal component analysis (PCA), PCA score plot can clearly distinguish the differences of the different blood sampling time chromatogram, caused by partial least squares (PLS) method can further found Gegenqinlian into blood components change over time, thus contributing reveal the timeliness Gegenqinlian relationship. By liquid chromatography with diode array and mass spectrometry combined with other methods, the Gegenqinlian serum drug chemical research, chemical composition in rat plasma after oral Gegenqinlian separation of ownership and identification, and to build a plasma The pharmacokinetics fingerprint clarifying of certain substances in the body's metabolism and its dynamic process of change, new ideas and methods to provide for the the Chinese medicines substance basic research.
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