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Objective: hepatic fibrosis is a high incidence of the disease , the drugs can effectively treat or reverse liver fibrosis . In recent years , studies have shown that traditional Chinese medicine has a good anti-hepatic fibrosis . In for particles of veitchiorum preliminary studies found experimental animal liver fibrosis liver tissue has a protective effect , and has antifibrotic . SD rat model of hepatic fibrosis induced by dimethylnitrosamine (dimethylnitrosamine, DMN) In this study , the detection of hepatic serum markers ALT , AST , ALB levels and liver tissue α-SMA, MMP-2 expression to explore the particle therapy veitchiorum mechanism of liver fibrosis . Methods: SD 48 female rats were randomly divided into normal group , model group , the Blue Hosta particles , and small dose group of γ- interferon group . In addition to the normal group , DMN10mg/kg intraperitoneal injection of the remaining rats injected once every other day for 30d . 31 days, abdominal aortic blood animals in each group were sacrificed , the rapid removal of the liver , the right lobe of the liver made ??the tissue homogenates biochemical detection , the left lobe of the liver invested fixed in 10% formaldehyde , the conventional embedded , sliced ??, HE staining on liver histopathological changes were observed under the optical microscope . Serum alanine aminotransferase (ALT), aspartate aminotransferase ( AST ) , albumin (ALB) Determination ; liver homogenates α- smooth muscle actin ( alpha -SMA ) , matrix metalloproteinase -2 (MMP- 2) Determination . Results: The model serum ALT, AST was significantly higher , ALB significantly reduced ; significantly increased liver homogenates of α-SMA , MMP-2 decreased compared with normal group there was a significant difference ( P <0.01 ) ; Blue Hosta large particles , small dose treatment group compared with model group , serum ALT, AST reduce , ALB increased ( P <0.01 ) ; reduced α -SMA , MMP-2 activity in liver tissue increased ( P <0.01 ) , there are very significant difference ; compared with the model group, MMP-2 level in addition to a the blue the Hosta particles small dose group , the difference was not statistically significant . HE staining observed significant improvement in the degree of liver fibrosis . Conclusion : veitchiorum particles by reducing ALT, AST, increased content of ALB to reduce the inflammation of the liver , protect the liver function ; reduce α -SMA , elevated MMP-2 activity , inhibit or reduce the formation of DMN -induced liver fibrosis .
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