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Establishment of Polygonum cuspidatum on fingerprints , use the the the map evaluation 10 origin cuspidatum quality of medicine , its HPLC fingerprint analysis and the use of chromatographic fingerprint similarity evaluation system (2004A ) using high-performance liquid chromatography , ultraviolet spectroscopy and infrared spectroscopy provide a reference , for the of Polygonum cuspidatum medicinal quality evaluation . From Polygonum cuspidatum extract the separation polysaccharide , and structure and in vivo , in vitro biological activity in preliminary studies, to provide a reference basis for the development of Polygonum cuspidatum polysaccharide products . Polygonum cuspidatum crude polysaccharide passes through in addition to protein , to the pigment , and purified by DEAE -52 column and a SephadexG - 200 column to give a purified polysaccharide (named pCp Ⅰ ) , to meet the requirements of structural identification HPLC method to verify its purity . Comprehensive analysis by gas chromatography, infrared spectroscopy , and chemical analysis , characteristic absorption peaks the PCP Ⅰ having polysaccharide , the monosaccharide composition is glucose . Vitro hepatoprotective test , mice immune tests , antibacterial and analgesic trials , the Polygonum cuspidatum polysaccharide has good antioxidant role . When the concentration of 1mg/mL lipid peroxidation inhibition rate reached 52.45% ; enhancement of related enzymes in the body (T-AOC, SOD) activity , increased spleen index ; also has a significant analgesic effect . The establishment of Polygonum cuspidatum herbs HPLC, UV and IR fingerprint spectra can be applied to evaluate the authenticity of Polygonum cuspidatum herbs , good and stability , can better the quality of the evaluation cuspidatum herbs . Polygonum cuspidatum polysaccharide has hepatoprotective enhanced immunity and analgesic . Of Polygonum cuspidatum polysaccharide PCP Ⅰ glucose polymerization , for the same polysaccharide .
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