|
The first part immunohistochemistry identification purposes of SOCS3 expression in normal and Abortion: a preliminary understanding of normal and Abortion SOCS3 expression Bushen Yiqi To explore how the basis of immune regulation on decidual do. Methods: 6-10 weeks gestation artificial abortion normal pregnant women and unexplained abortion decidua of pregnant women using immunohistochemical methods to detect normal and abortion decidua whether expression of SOCS3. Results: In normal pregnancies and abortion decidua, both glands or interstitial can be seen SOCS3 expression. Conclusion: normal and Abortion has SOCS3 expression, which provides a possible mechanism for kidney Qi parties to further explore how regulation of the decidual the immune balance. The second part of the kidney Qi Fang human decidual CD4 ~~ T cell secretion of Th1 and Th2 cytokines Objective: To observe the kidney Qi Fang human decidua CD4 ~ T cells to secrete IL-4, IL-10 and IFN -γ, TNF-α, regulate, investigate its regulation maternal-fetal interface the Th1/Th2 immune miscarriage mechanism. Methods: 40 adult female SD rats were randomly divided into two groups: kidney Qi Fang and saline groups, respectively, the same volume of traditional Chinese medicine and saline orally, morning and evening, three days carotid blood; collection progesterone 6 -10 weeks induced abortion decidua of normal pregnant women, and by means of immunomagnetic beads isolated CD4 ~ T cells were cultured and divided into four groups: 10% fetal bovine serum, normal control group (A), 10% of Chinese medicine serum group (B), 20% decoction group (C), 10% of the animals fed with saline serum group (D), were cultured 24h, 48h, 72h, ELISA for detection of cell culture supernatant IL -4, IL-10 and IFN-γ and TNF-alpha level of. Results: group B and group C compared with group A, Th2-type cytokines IL-4, IL-10 water were significantly increased (P <0.05), Th1-type cytokines IFN-γ levels were significantly decreased (P < 0.01). Between the results of the group B and group C without significant differences; Group B 24h period of TNF-α and group A compared to meaningful (P <0.05). Conclusion: kidney Qi Fang promote decidual CD4 ~~ T secrete IL-4, IL-10, and inhibit its produce IFN-γ and TNF-α, maternal-fetal interface so as to promote the formation of Th2 dominant. The SOCS3 gene third part of the kidney Qi Fang CD4 ~ T cells in human decidual impact Objective: To investigate whether the kidney Qi Fang by SOCS3 gene regulate the differentiation of Th cells to explore the role of the Kidney Qi Fang regulation Th cell differentiation mechanism. Methods: 40 adult female SD rats were randomly divided into two groups: kidney Qi Fang and saline groups, respectively, the same volume of traditional Chinese medicine and saline orally, morning and evening, three days carotid blood; collection progesterone 6 -10 weeks induced abortion decidua of normal pregnant women, and by means of immunomagnetic beads isolated CD4 ~ T cells were cultured and divided into four groups: 10% fetal bovine serum, normal control group (A), 10% of Chinese medicine serum group (B), 20% decoction group (C), 10% of the animals fed with saline serum group (D), the cells were collected after 24 hours incubation, real-time PCR to detect groups SOCS3 gene transcription status , western blot detection 24h groups SOCS3 protein expression status. Results: ① realtime FCR: Compared with group A, group B and D group no difference, while Group C SOCS3 mRNA in the amount significantly rose (P <0.01); ② The western blotting was: compare each group with the ratio of the internal reference to evaluate each group proteins The ratio of the expression level, as compared with group A, B and C groups were statistically significant (P <0.05), and wherein the C group is more significant changes (P <0.01). Conclusion: C SOCS3 mRNA levels increased the kidney Qi Fang group SOCS3 protein expression was significantly increased, to kidney Qi may by SOCS3 to regulate the differentiation of Th cells.
|