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Yellow baicalensis glycosides (baicalin) is one kind of flavonoid class compound extracted from Scutellaria dried root Scutellaria main active ingredient, one of its antipyretic role significantly at the same time due to baicalin have good antibacterial role, can reduce the combined antibiotics consumption, reducing its toxicity, even some resistant bacteria into susceptible strains, compared with antipyretic western medicine baicalin has its unique advantages, much of the attention of the people. The fever is clinically more of the common symptoms of the disease, is a multi-link, multiple factors involved in the complex process, which some of the factors that link is suppressed or blocked, you can control body temperature rises, resulting antipyretic effect. Baicalin antipyretic mechanism is not yet clear, the whether baicalin affect the endogenous pyrogen generate has been reported. This paper copy yeast febrile rat model, baicalin antipyretic effects in rats, and by measuring the rat hypothalamus, IL-1β, TNF-α and IL-6 levels in the cerebrospinal fluid and serum baicalin to explore antipyretic effects of endogenous pyrogen (Endogenous pyrogen, EP) relations; using RT-PCR assay in rats of hypothalamus of IL-1βmRNA the expression, to further explore baicalin play antipyretic effects by influencing the transcription of IL-1β and inhibit its synthesis. Baicalin yeast-induced fever in rats antipyretic effect of 20% dry yeast suspension 2 g · kg -1 dose elect a group of rats as control rats subcutaneously (sc) injection, every 2 h measuring body temperature once measured continuously 14 hours, observed in each group changes in body temperature and changes in body temperature curves. Model group rats were injected yeast suspension 6h after the onset of fever, the 8h rectal temperature reached a peak, fever continued to 14h, the △ T up to 1.50 ° C, compared with the control group, there was significant difference (P lt; 0.01). The model is more stable, long duration of fever, good reproducibility, easy to master. Fever rat model of replication ibid., observed that oral administration of baicalin (160mg · kg-1, 80mg · kg-1, 40 mg · kg-1) antipyretic effects in rats. The results showed: pyrogenic after 6 h, the 8h baicalin high dose (160mg · kg-1) and dose (80mg · kg-1) group compared with the model group, both fever magnitude lower (P lt; 0. 01), and a dose-dependent, the baicalin low-dose group △ T model group showed no statistical significance (P gt; 0.05); fever stage after each dose group baicalin the fever magnitude compared with the model group were significantly reduced. Above baicalin on the process of yeast-induced fever suppression and intervention. 2 baicalin endogenous pyrogen and antipyretic effect copy yeast-induced fever in rats model, observed that oral administration of baicalin (160mg · kg-1, 80mg · kg-1, 40 mg · kg-1) of the antipyretic effect in rats, using double antibody sandwich ELISA fever peak rat hypothalamus, cerebrospinal fluid and serum IL-1β, TNF-α and IL-6 levels, and its antipyretic effect with EP the relationship between the regression analysis. Baicalin (160mg · kg-1) can significantly reduce the serum, hypothalamus and cerebrospinal fluid IL-6, IL-1β, TNF-α content. Correlation analysis showed that baicalin caused by the temperature change rate with IL-1β content change rate was positive related (r = 0.8726, P lt; 0.05), and TNF-α content change rate also showed a positive correlation (r = 0.8624, P lt; 01), but the rate of change of the IL-6 correlation (r = 0.3794, P gt; 0.05). In addition, baicalin inhibited rat body temperature changes with serum (r = 0.8838, P lt; 0.01), hypothalamus (r = 0.6734, P lt; 0.05) in three cytokines content was positively correlated with cerebrospinal fluid in cytokine content changes without correlation (r = 0.3521, P gt; 0.05). The baicalin above tips are obvious antipyretic effect, its mechanism of action may be mainly associated with reduced peripheral and hypothalamic content of TNF-α and IL-1β. Baicalin endophytic induced pyrogen of IL-1βmRNA expression of rats were randomly divided into control group, model group, baicalin group, observed changes in body temperature after the drug; using RT-PCR assay in rats with fever peak hypothalamic interleukin prime-1β (IL-1β) mRNA expression. The results showed the baicalin group compared with the model group rats hypothalamus of IL-1βmRNA the expression was significantly reduced (P lt; 01). Indicating that the baicalin antipyretic effect may be related to the inhibition of transcription of IL-1βmRNA affect its synthesis.
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