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Objective: comparison of eosinophils in asthma (eosinophil asthma, EA) and the non eosinophils asthma (non-eosinophil asthma, NEA) the difference in the inflammatory cells and cellular components and characteristics; bronchial asthma TCM the Han Tan \Methods: inclusion criteria: age 18-70 years, limited to gender, modern medical diagnosis of bronchial asthma; installments exacerbation of chronic persistent and acute; the TCM Hantan card, the hot Phegm and non-the Han Tan heat sputum permits patients; another select age and sex-matched healthy people with no respiratory disease as a normal person. Exclusion criteria: with pulmonary disease, nearly two weeks in patients with respiratory infections and allergic diseases. TCM into the Han Tan syndrome group group of hot Phegm and non-Han Tan hot phlegm syndrome group and the normal group, 30 cases in each group. All subjects so by induced sputum coughed up sputum, sputum smear counts of eosinophils (eosinophil, EOS) percentage (EOS), neutrophil (Polymorphonuclear Neutrophils, PMN) percentage (PMN%), sputum EOS% divided into the EA group (EOS% ≥ 2%), NEA group and normal group. Routine blood test measuring PMN% EOS%; enzyme-linked immunosorbent assay was used to detect sputum supernatant and serum interleukin (interleukin, IL) -8, IL-5, eosinophil cationic protein (eosinophil cationic protein, ECP), IL-4, IL-6, IL-10, of leukotrienes (Leukotriene LT) B4, C-reactive protein (C-reactive protein, CRP), interferon (interferon, IFN)-γ levels; detection of patients with asthma lung function FEV1,%, FEV1/FVC%. Results: The general information in this study were collected 90 cases of patients with asthma and the Han Tan Certification Section 27 cases, the hot Phegm group of 32 patients, non-the Han Tan heat Phegm group 31 cases. All patients underwent induced sputum cell count, 83 cases of induced success, sputum EOS% increased in 33 cases (40%), 60% of asthma patients showed NEA. Patients with elevated blood EOS% of the 38 cases (46%), the blood PMN% in patients with elevated three cases (4%). Changes in indicators of the EA group, NEA group and normal group. Groups sputum and blood of the EOS% of PMN% change: EA group blood and sputum PMN% with normal group was no significant difference (P gt ; 0.05), blood and sputum EOS% compared with normal group was significantly higher (P lt; 0.01); NEA group blood and sputum PMN% EOS% with normal group had no statistical difference (P gt; 0.05) . EA group sputum PMN% lower than the NEA group, blood and sputum EOS% was significantly higher than the NEA group (P lt; 0.05). 2 serum and sputum supernatant inflammation indicators of change: the EA group sputum supernatant IL-8, IL-4, IL-5, IL-10/IL-6, of IFN-γ/IL-4 with normal group no significant difference (P gt; 0.05)), ECP (Extended Capabilities Port, LTB4, IL-6, IL-10, IFN-γ water were higher than those in the normal group (P lt; 0.05); the NEA set of sputum supernatant IL -8, IL-6, IL-5 with the normal group had no statistical difference (P gt; 0.05), ECP, IL-10, IFN-γ, the LTB4 level of, IL-10/IL-6, IFN-γ / IL-4 values ??were higher than the normal group (P lt; 0.05), IL-4 levels lower than normal group (P lt; 0.05). The EA group sputum supernatant IL-5, IL-6 levels above the NEA group (P lt; 0.05), IL-8, ECP, IL-4, IL-10, IFN-γ, of LTB4 in the level of IL-10 / IL-6, IFN-γ/IL-4 with NEA group had no statistical difference (P gt; 0.05). The EA group serum of LTB4 in CRP water on average significantly higher than the normal group (P lt; 0.01), IL-5, IL-8, IL-4, ECP, IL-6, IL-10, IFN-γ, IL-10 / IL-6, IFN-γ/IL-4 normal group had no statistical difference (P gt; 0.05); NEA serum IL-5, LTB4, CRP level higher than the normal group (P lt; 0.05), IL -8, IL-4, ECP, IL-6, IL-10, IFN-γ, IL-10/IL-6, of IFN-γ/IL-4 normal group, there was no statistically significant difference (P gt; 0.05 ). EA serum indicators NEA group had no statistical difference (P gt; 0.05). Comparison of lung function 3.EA group and NEA: NEA group FEV1 percent predicted value% and FEV1/FVC% values ??were significantly higher than the EA group (P lt; 0.01). , The Han Tan syndrome group, the hot Phegm group, non-the Han Tan hot Phegm group and normal group indicators change each group of blood and sputum the EOS% of PMN% comparison: hot Phegm group sputum PMN % higher than the normal group and the non-the Han Tan heat phlegm syndrome group (P lt; 0.05), phlegm and blood of the EOS%, blood PMN% normal human groups and non-the Han Tan hot Phegm group showed no significant difference (P gt ; 0.05); the Han Tan Certification Section blood and sputum the EOS% were higher than the normal group, non-the Han Tan heat Phegm group and thermal Phegm of (P lt; 0.01), blood and sputum of PMN% with other groups compare No statistically significant difference (P gt; 0.05); non the Han Tan hot Phegm group sputum and blood of the EOS% PMN% with normal group had no statistical difference (P gt; 0.05). Comparison of blood and sputum supernatant inflammatory markers: of hot phlegm syndrome group sputum supernatant IL-10, IL-10/IL-6 value higher than the normal group (P lt; 0.01), IL- 8, IL-5, ECP, IL-4, IL-6, IFN-γ, LTB4, IFN-γ/IL-4 with normal group had no statistical difference (P gt; 0.05); Han Tan syndrome group sputum supernatant ECP, LTB4, IFN-γ water were higher than those in the normal group (P lt; 0.05), IL-8, IL-5, IL-4, IL-6, IL-10, IFN-γ/IL -4, IL-10/IL-6 no significant difference with the normal group (P are gt; 0.05); non-the Han Tan heat phlegm syndrome group sputum supernatant ECP, IL-6, IL-10, IFN- the γ, IL-10/IL-6 average higher than the normal group (P lt; 0.05), IL-8, IL-5, IL-4, of IFN-γ/IL-4, LTB4 with normal controls No statistically significant difference (P gt; 0.05). Group sputum supernatant of the hot Phegm group and Han Tan card indicators the water average non the Han Tan hot Phegm group without statistical differences (P gt; 0.05). The Han Tan syndrome group the sputum supernatant IL-10/IL-6 value higher than the hot Phegm group (P lt; 0.05), IL-8, IL-5, ECP, IL-4, IL-6, IL-10 , IFN-γ, IFN-γ/IL-4, LTB4 water average heat phlegm syndrome group no significant difference (P gt; 0.05). Hot Phegm serum IL-5, the average CRP, LTB4 water higher than the normal group (P lt; 0.05), IL-8, IL-6, IL-10, IFN-γ, ECP, IL-4, of IL- 10/IL-6 of IFN-γ/IL-4 value of the normal group showed no significant difference (P gt; 0.05); the Han Tan syndrome group IL-5, IL-4, the average CRP, LTB4 water higher than normal people group (P lt; 0.05), ECP the level, IFN-γ/IL-4 values ??were lower than the normal group (P lt; 0.05), IL-8, IL-6, IL-10, IFN-γ, IL -10/IL-6 non-Han Tan the hot Phegm group serum IL-5, CRP of LTB4 in level, IFN-γ/IL-4 values ??were higher than the normal group, there was no statistically significant difference (P gt; 0.05); normal group (P lt; 0.05), ECP, IL-4, IL-8, IL-6, IL-10, IFN-γ, IL-10/IL-6 level of the normal group showed no statistically significant difference ( P gt; 0.05). Higher than non-the Han Tan heat phlegm syndrome group (P lt; 0.05) the Han Tan securities serum IL-4 levels of IFN-γ/IL-4 value is significantly lower than the non-the Han Tan heat phlegm syndrome group (P lt; 0.01) IL-8, IL-5, ECP, IL-6, IL-10, IL-10/IL-6, CRP, LTB4, the level of IFN-γ and non-the Han Tan heat phlegm syndrome group had no statistical difference (P gt; 0.05); the heat Phegm serum IFN-γ levels, IFN-γ/IL-4 values ??were lower than the non-the Han Tan heat phlegm syndrome group (P lt; 0.05), serum IL-4, IL-8, IL-5 , ECP, IL-6, IL-10 IL-10/IL-6, CRP, LTB4 level of non-Han Tan hot phlegm syndrome group was no significant difference (P gt; 0.05). The the the Han Tan serum indicators are with hot Phegm group was no significant difference (P gt; 0.05). 3. Lung function of the comparison the the Han Tan syndrome group, hot Phegm group of non-Han Tan the thermal Phegm group: the Han Tan certificates groups FEV1 percent predicted value% value significantly lower than that the non the Han Tan heat Phegm group and heat Phegm of group ( P lt; 0.01); FEV1/FVC% value lower than the hot Phegm group (P lt; 0.05), but no significant differences (P gt with non-the Han Tan heat Phegm group; 0.05). The hot Phegm group FEV1 expected value% and FEV1/FVC% value of non-Han Tan hot phlegm syndrome group had no statistical difference (P gt; 0.05). Serum and sputum supernatant indicators results of the analysis of blood and sputum EOS% has a significant positive correlation (r = 0.585, p = 0.000), sputum and blood PMN, IL-8, IL -5, ECP, IL-4, IL-6, IL-10, IFN-γ, LTB4 was not significant (r = -0.002, -0.016, -0.003, -0.023,0.012,0.017, -0.087 , -0.083,0.137, P gt; 0.05). Sputum EOS sputum supernatant IL-6, IL-5, serum of LTB4 and serum CRP level was a significant positive correlation (r = 0.224 p = 0.032; r = 0.227 p = 0.036; r = 0.258 p = 0.016), respectively FEV1,% showed a significant negative correlation (r = -0.234 p = 0.035), with sputum supernatants IFN-γ, ECP, IL-8, IL-10, IL-4, LTB4, of serum IL-4, IL -5, IL-6, IL-10, IFN-γ, IL-8, ECP and lung function between FEV1/FVC% is not significant (P gt; 0.05); sputum PMN with sputum supernatant fluid IL-6, IL-8 level was a significant positive correlation (r = 0.221 p = 0.035; r = 0.214 p = 0.034), with sputum supernatants IFN-γ, ECP, IL-10, IL-5, LTB4 correlation between IL-4 and indicators in the serum and lung function significantly (P gt; 0.05); blood the EOS with sputum supernatant in the level of IL-5 was a significant positive correlation (r = 0.344 p = 0.001 ), and lung function FEV1 accounted for the expected value% and FEV1/FVC%, respectively, showed a significant negative correlation (r = -0.471, p = 0.000; r = -0.255 p = 0.023), and sputum supernatant IFN-γ, ECP, IL -10, LTB4, IL-4, IL-6, IL-8, the correlation between serum indicators significantly (P gt; 0.05); blood PMN and other indicators are not significant ( P Asthma airway inflammation and systemic inflammation. Sputum and peripheral blood EOS proportion of positive correlation, and other inflammatory cytokines in the serum and sputum no obvious correlation, not entirely consistent airway local inflammation and systemic inflammation both sputum better than serum reflect asthma inflammatory characteristics. The essence of Chinese sputum is inflammation, while the essence is anti-inflammatory and expectorant. The asthma the Han Tan permit type of inflammation characterized mainly increased for the EOS, the the hot Phegm performance local airway PMN increased sputum the EOS% and PMN% indicators asthma Han Tan, the hot Phegm microscopic proof is an important indicator. Asthma the Han Tan permit type may be similar to the characteristics of airway inflammation with EA, and the performance of the dominant TH2 type cytokines. We think the Han Tan permit the essence of eosinophils in asthma, the nature of the heat phlegm infectious inflammation. Treatment, we advocate the \Patients identified as \This study provides a theoretical basis for the application of this rule is.
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