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Objective: To investigate Bu Yang Huan Wu Tang to improve sepsis rat spleen immune function, provide evidences and clues for Integrative Medicine sepsis. Methods: 180 healthy male Wistar rats, divided into sham group, model group, Bu Yang Huan Wu Tang (Buyanghuanwutang, BYHWT) treatment group and BYHWT treatment group by random number table. Animals in each group were sacrificed point in time (of 6,12,24,48,96 hour) and then divided into five teams each. Copy a rat model of sepsis by cecal ligation and puncture (cecal ligation and puncture, CLP). Animal model of BYHWT treatment group given daily orally Bu Yang Huan Wu Tang BYHWT control animals preoperative given Bu Yang Huan Wu Tang orally. Each group animals the appropriate point in time after the abdominal vein blood after the animals were killed spleen. Endotoxin detector detects animals in each group at each time point serum endotoxin levels, using the the HE staining optical microscope observation groups morphological changes in the spleen of experimental animals the immunohistochemistry assay animals in each group at each time point spleen the CD3 ~~ T, CD4 ~~ T, CD8 to T-lymphocytes and B lymphocytes level, TUNEL assay rats in each group at each time point spleen apoptosis. Application image pro plus 6.0 image analysis system for image acquisition and analysis. Results: CLP rats after spleen visible in the light microscope the white pith gradually atrophy, lymphoid nodules destruction, the severity of this performance model group> BYHWT, the treatment group> BYHWT control group. TUNEL staining of apoptotic cells first appeared in the splenic periarterial lymphatic sheath then also visible. After CLP model the group, BYHWT treatment group, BYHWT control group than in the sham group at each time point serum endotoxin levels of rats was significantly higher (p <0.01); 24 hours after the start, BYHWT treatment serum endotoxin levels that significantly lower than the model group (p <0.01); 12 hours after the operation began, BYHWT control serum endotoxin levels that was significantly lower than that in the model group (p <0.01); 24 hours after the start BYHWT control serum endotoxin levels that lower than BYHWT treatment group (p <0.05). 6 hours after CLP started model group, BYHWT treatment group, BYHWT, control rats spleen CD3 ~ T, CD4 ~ T lymphocyte and B lymphocyte levels significantly lower than in the sham group (p <0.01), while each group CD8 ~ T lymphocytes compared with sham-operated group, has always been no significant change (p> 0.05); the CLP 24 hours after the start, BYHWT treatment group rat spleen CD3 ~ T, CD4 ~ T lymphocytes and B lymphocytes were significantly higher than the model group (p <0.01); CLP postoperative 6 Xing stupid? BYHWT control rats spleen CD3 to T, the CD4 ~~ T lymphocytes and B lymphocytes were higher than the model group (p <0.01 or p < 0.05); six hours after the operation began, rat spleen BYHWT prevention group CD3 to T, CD4 ~~ T lymphocytes and B lymphocytes water average to higher than BYHWT treatment group (p <0.01 or p <0.05). After CLP model the group, BYHWT treatment group, BYHWT treatment group rat spleen cell apoptosis at each time point compared with sham group was significantly higher (p <0.01); six hours after the start, BYHWT treatment group and BYHWT prevention group spleen cells from apoptosis level that is significantly lower than the model group (p <0.01); six hours after the start of, BYHWT Combat spleen apoptosis level that is lower than BYHWT treatment group (p <0.05). Conclusion: septic rats serum endotoxin levels significantly increased spleen CD3 ~ T, CD4 ~ T lymphocytes and B lymphocytes decrease in the number of significant increase in spleen apoptosis; 2, BYHWD can effectively reduce septic rats serum endotoxin levels, reduce spleen apoptosis, increase the CD3 ~~ T, the of of CD4 ~~ T lymphocytes and B lymphocytes number; 3, the effect of treatment of septic rats, BYHWD prophylaxis is superior to simple therapeutic medication.
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