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Objective: To study shows that a clear upward trend of the prevalence of metabolic syndrome, and cardiovascular disease is the final result of the development of the metabolic syndrome, the occurrence of cardiovascular disease, the mortality rate is also significantly higher. Known to play an important role in the mechanism of oxidative stress in cardiovascular disease, and patients with metabolic syndrome as a cardiovascular disease in high-risk populations, but little is known about the relationship between antioxidant nutrients and. The purpose of this study is to explore the metabolic syndrome among the major antioxidant nutrients and related biochemical indicators of the patient's body, to provide scientific information for the prevention and treatment of metabolic syndrome. Method: ① for the simultaneous determination of serum vitamin A, E, lycopene and β-carotene by high performance liquid chromatography (HPLC), vitamin A acetate as internal standard. The column was Hypersil ODS (4.6 × 250 × 5μm); mobile phase A methanol: acetonitrile: water (V: V: V) = 6:2:2, the mobile phase B was acetonitrile: methanol: tetrahydrofuran (V: V: V) = 5:2.5:2.0; elution procedures see the first part of Table 1-1; flow rate: 1ml/min; injection volume: 20 μl; Column temperature: 45 ℃ detection; wavelength: vitamin A and vitamin A acetate 325nm, 292nm of vitamin E, beta-carotene 450nm, lycopene 472nm. (2) observation of patients with metabolic syndrome, the relationship between anti-oxidation of nutrients and related biochemical indicators. A total of 60 pairs after age and sex paired metabolic syndrome patients with non-metabolic syndrome serum vitamin A, E, Fan lycopene (LP), beta-carotene (beta-C), fasting glucose (Glu), serum total cholesterol (TC), serum triglyceride (TG), high density lipoprotein (HDL-C) and other indicators, the use of the t test, single factor correlation analysis, stepwise multiple linear regression method for medical statistical analysis. In results: ① Vitamin A, vitamin A acetate, vitamin E, beta-carotene, lycopene retention time are 3.7,4.2,6.8,8.3,10.5 min; vitamin A recovery rate of 91.0% to 95.8% vitamin E recovery of 98.0% to 86.4%, from 105.0% to 97.6% recovery of beta-carotene, lycopene recovery was 117.0% to 106.4%; vitamins A, E, β-carotene, tomatoes red pigment day RSD were: 3.47%, 3.94%, 17.91% and 5.80%, vitamin A, E, beta - carotene, lycopene day RSD: 3.54%, 3.89%, 22.28% and 7.78% ; linear range of the standard curve: Vitamin A 0.14 ~ 7μg/ml, vitamin E 100μg/ml, beta-carotene and lycopene 0.04 ~ 2μg/ml; 60 normal human serum samples detection results The vitamin A content of 0.624 ± 0.254μg/ml; vitamin E content of 12.095 ± 4.125μg/ml; beta-carotene content of 0.188 ± 0.085μg/ml; lycopene content of 0.378 ± 0.244μg/ml. ② metabolic syndrome serum triglycerides (2.29 ± 1.37mmol / L) was significantly higher (P lt; 0.01), beta-carotene content (0.282 ± 0.193μg/ml) was significantly lower than the control group, the difference significantly (P lt; 0.05). ③ single factor Pearson correlation analysis showed that serum triglycerides and β-carotene was negatively correlated (P lt; 0.01), multivariate linear stepwise regression analysis showed that serum triglycerides and β-carotene showed significantly negative correlation (P lt; 0.01). Conclusion: HPLC internal standard method for determination of serum vitamin A, E, lycopene and β-carotene content method is reliable, fast and easy sample handling. Metabolic syndrome antioxidant nutrients and biochemical markers, serum triglycerides decreased with the increase in the level of serum β-carotene.
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