Dissertation > Excellent graduate degree dissertation topics show

Analysis of the Safety in Drinking Water Which Can Be Bought in the Market by Using Luminiscent Bacteria

Author: ZhangYaDan
Tutor: XuYaTong
School: East China Normal University
Course: Environmental Science
Keywords: Early warning system Luminescent bacteria Qinghai Vibrio Joint toxicity Additive effect Antagonistic effect Drinking water
CLC: R155.5
Type: Master's thesis
Year: 2009
Downloads: 235
Quote: 3
Read: Download Dissertation

Abstract

Drinking water security is directly related to the health of the human body. With the development of industry and science and technology, chemicals contamination of drinking water has drawn increasing attention. According to a survey conducted by the foreign, in drinking water has identified hundreds of chemicals, most of which is harmful to the human body, so research can quickly determine whether drinking water toxicity detection methods of today's domestic and foreign research hotspot. Traditional the biological toxicity evaluation of mammals, fish and other subjects, the complex experimental operation, long life cycle, and only suitable for use in the laboratory, can not meet the needs of the fast monitoring. In recent years, researchers at home and abroad have been committed to the exploration and research of new biological toxicity test method. This thesis is based on bacterial luminescence detection the toxicity and biostatistics principle, the establishment of a rapid detection method of drinking water biological toxicity. In this paper, luminescent bacteria light-emitting principle, Qinghai vibrios (Vibrio qinghaiensis sp.nov) as a toxicity test species, secondary two, three and four-factor regression general rotation combination of experimental design and statistical methods, as well as adding the index of heavy metal , pesticides and other poisons, such as drinking water contaminants a single toxicity and joint toxicity studies, reveals the total toxic effect of the coexistence of multiple pollutants, the role of the interaction and the main factor is constructed with a mixture of the total biological toxicity mathematical model; initially established a luminous Bacteria rapid evaluation of the safety of drinking water system, including the the bioluminescent bacteria bottled drinking water, bottled mineral water for drinking fast security assessment method; rapid detection of the system conforms to the safety of drinking water integrated demand services to improve drinking water safety warning system and supervision and inspection system. The main conclusions of the research are as follows: 1 study of heavy metals and 11 pesticides, the acute toxicity of a single pollutant luminescent bacteria in a certain concentration range, the relative luminescence intensity was negatively correlated with the concentration of pollutants, related different the multiple coefficient greater than 0.84. 2 different concentrations of heavy metals mixture of joint toxicity of luminescent bacteria. Based on the test results, respectively fitting 17 relative emission rate and concentration of heavy metal compounds regression model tested significantly (P lt; 0.01) and the fitting effect (R = 0.865 to 0.985). Under our experimental conditions, multiple joint toxic effects of heavy metals in the mixture and their concentration range. Multi-pesticide mixture of three different concentrations of the toxic effects of Qinghai Vibrio. Using statistical methods to study the joint toxicity of binary pesticide mixture of luminescent bacteria, respectively, fitting a regression model of two relative luminous efficiency of pesticide concentrations the efficient cypermethrin and Monosultap of (R = 0.652) fitting effect general; regression model oxadixyl and dichlorvos (R = 0.926), fitting better. In our experimental conditions, the use of the additive index method 8 group combined with the toxic effects of pesticides mixed system, the results are antagonistic activity. Were detected 11 species, 10 species, nine species, eight kinds, seven kinds, six kinds of toxic effects of the five kinds of pesticides minimum detection limit of the mixture of luminescent bacteria. The results showed that the mixture was diluted smaller the greater the degree of toxicity of Qinghai Vibrio. The 4 potassium cyanide, the acute toxicity of arsenic trioxide on the Qinghai Vibrio. In a certain concentration range, Qinghai Vibrio relative luminous intensity toxicant concentration was negatively correlated with a correlation coefficient greater than 0.9. Use statistical methods to study the toxic effects of potassium cyanide, arsenic trioxide, potassium dichromate and copper chloride the four yuan mixture in different time of Qinghai Vibrio. The results showed that the fitting effect (correlation coefficient of 0.586 to 0.850). Melamine and microcystin Vibrio luminous intensity of Qinghai respectively, the results show that, in the role of 15min after a certain amount of melamine and microcystins significantly inhibited the luminous intensity. 5 Drinking Water Standards (GB 5749-2006) Qinghai detection of Vibrio Q67, limit detection of 14 substances formulated mixture toxicity at different pH values ??and different dilutions glow luminescent bacteria inhibition kinetic curves. The study showed that 14 kinds of restrictions detection substances, although their concentrations are in line with GB limit concentration value, but they coexist joint toxicity is very significant, and may be harmful to life and human. 6 on the basis of previous work, by building the relationship between drinking water safety evaluation grade 15min relative emission rate, initial establishment of bioluminescent bacteria safe drinking water rapid evaluation system, including bottled drinking water of bioluminescent bacteria Bottled drinking mineral water security rapid assessment method. 5 bioluminescent bacteria is a rapid biological test used for joint multi-system toxicity studies. It can quickly evaluate the biological toxicity of drinking water, contaminated drinking water screening can produce a variety of toxic and hazardous substances coexist biological toxicity evaluation of potential risk factors in the drinking water for biological toxicity analysis, real-time detection, pre-treatment is simple, low cost advantages, in qualitative and semi-quantitative rapid field detection gradually showing its advantages, will be a new area of ??development of modern drinking water safety monitoring technology .

Related Dissertations

CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Food hygiene and food inspection > Food hygiene and inspection
© 2012 www.DissertationTopic.Net Mobile