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Single Molecule Spectral Analysis of Gold Nanoparticles and Gold Nanorods Using Dark Field Microscopy

Author: SongYuanYuan
Tutor: HeYan
School: Hunan University
Course: Analytical Chemistry
Keywords: Localized surface plasmon resonance Gold nanoparticles Gold nanorods Dark field microscopy Raster
CLC: TB383.1
Type: Master's thesis
Year: 2009
Downloads: 528
Quote: 4
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Abstract


Metal nanoparticles localized surface plasmon resonance (LSPR) can show unique optical absorption and scattering properties, such as particle shape and vulnerable LSPR size spectrum, their environment, the linker molecule specific recognition and subsequent other factors. It is precisely because of the surfaces of metal nanoparticles can cause subtle changes in the spectrum changes LSPR, which is often used for physical, chemical and biological analysis and detection areas. Among them, the gold nanoparticles With simple preparation, easy modification, stability and biocompatibility and good LSPR spectroscopy applications and become the main research goals. In recent years, with the spectral sensitivity and imaging technology, and at the single cell level, nanoscale single time and in situ study of metal nanoparticles with the surrounding environment and cell interactions increasingly become a hot research. Single metal nanoparticles LSPR spectra usually dark-field microscope, but the existing dark field microscopy - spectrometer combined with a quiescent state apparatus can only get a single metal nanoparticle LSPR spectra and dark-field images, can not achieve high throughput detection as well as real-time tracking trajectory samples, and costly. In this paper the problem, combined with the transmission grating optical properties and dark-field microscope imaging principle, has designed a new, simple optical device can achieve a level of real-time two-dimensional, dynamic, high-throughput manner on metal nano-particles imaging study. Details are as follows: (1) individual gold particles proof experiment - the concentration gradient of the dark-field microscope in order to study the sensitivity and verify whether the general dark field microscope in a single 18 nm gold particles used in this experiment concentration gradient method their research. In obtaining a good fluorescent microspheres on the basis of the concentration gradient map, further optimization of the experimental conditions of this chapter, to solve the uneven distribution of gold particles, the dark field image of background noise is high. Final results of the 2000 ms exposure time, the modified glass slides in APTMS can be observed on a single 18 nm gold particles conclusions. This conclusion is to use dark field microscopy of individual gold nanoparticles LSPR spectroscopy provides a prerequisite. (2) gold nanoparticles real dark field spectroscopic imaging study established a new imaging techniques to enable them to live in a two-dimensional level in motion on multiple metal particles LSPR dark field imaging and spectroscopy. Used method is added in a dark field microscope grating plate having spectral effect, the gold particles into the image itself can display zero-order diffracted out of the level stripes and stripes, by determining the position and the two stripes strength of the granules can be obtained LSPR spectrum. And gold particles was found by the same motion state of the solution becomes adsorbed aminopropyl trimethoxysilane (APTMS) slides on a modified stationary state, the LSPR spectral values ??will change, which is because the metal LSPR spectra of nanoparticles by environmental change significantly. Therefore, the optical system to obtain a plurality of particles while the dark field image obtained LSPR its corresponding spectrum can be observed in real time and in which when the particles are caused by changes in the external environment of the LSPR spectral changes and track the process of particle motion trajectory. (3) gold nanorods darkfield spectral imaging fellowship stick solution inevitably there will be a certain percentage of spherical gold particles, and in the study on the gold bars which can not exist spherical gold particles separated. Using a dark field microscope coupled device (CCD) of the optical grating device preceded gold nanorod solution can be obtained in free motion of individual particles LSPR spectrum. Known gold nanorod LSPR peaks spherical gold particles with LSPR peaks significantly different, so by determining the particle LSPR spectroscopy can distinguish the solution of gold bars and spherical gold particles, the solution for the future separation of gold bars of gold particles provide preconditions. Meanwhile, the success of this technology for the separation of science provides a new research tool.

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