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Studies on the Biological and Mutagenic Effects of Salvia Splendens and Linum Usitatissmum Irradiated by 12C6+ Heavy Ions

Author: WuDaLi
Tutor: HouSuiWen
School: Lanzhou University
Course: Cell Biology
Keywords: A bunch of red Verbena Flax 12C6 heavy ion Biological effects Mutagenic effects and mechanisms Mutant
CLC: S565.9
Type: Master's thesis
Year: 2008
Downloads: 93
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Abstract


In this paper, a bunch of red, Verbena and flax as experimental material, in-depth study 12 C 6 heavy ion induced biological effects, mutagenic mechanism of heavy ion while filtering out the plurality of mutants. A detailed discussion of different doses and LET values ?? 12 C 6 heavy ion radiation of a bunch of red and dry seeds of Verbena studied irradiation on seed germination and seedlings impact. Low doses can increase the germination rate and gradually weakened as the dose increased, the catalytic role; LET irradiation of a specific range of strong inhibitory effect on seed germination and LET values ??smaller or larger its inhibition reduced. The seed seedling emergence Basically dose or LET value increases gradually postponed. The different doses LET values ?? 12 C 6 heavy ions like survival, seedling hypocotyl and cotyledon growth performance germination rate trends. In addition, a string of red and Verbena mortality rate of phenotypic variation and 12 C 6 LET values ??of the heavy ion irradiation has a very close relationship, the same a bunch of red apical cell chromosomes during mitosis distortion rate is also dependent on the 12 C 6 heavy ion irradiation LET values. Based on a hypothesis that low LET heavy ion value mainly caused chromosomal damage, high LET values ??mainly caused chromosomal damage (protein, cell membrane and organelles injury). The performance variability determined jointly by the chromosomal and non-chromosomal damage, so LET value increased, although the rate of chromosomal aberrations declined, but non chromosomal damage is rising, and eventually leading to the contemporary performance of a bunch of red mutation rate rise. Screened for a bunch of red combined frequency of survival and phenotypic variation, mutation breeding Verbena LET values ??and irradiation dose: a bunch of red for 50 keV / μm, 50 Gy; Verbena 150 keV / μm 100-500 Gy. 12 6 heavy ion irradiation, through multiple generations of continuous observation, selected from various types of mutant and three batches of a bunch of red suit chimera Leaf mutant were verified. A bunch of red chimera RAPD analysis show that has undergone great changes color and leaf mutant DNA obtained six new DNA sequence (Sasp1-6), and on this basis, to Sasp1-6 sequence to GenBank registration and registration number: EU363530, EU363531, EU363532, EU363533, EU363534 and EU363535. Verify the results also show no observed mutation effects in color chimera and leaf mutant offspring, indicating that the mutated gene may have been repaired. This is to verify that in later generations. The the 2. Consecutive generations on 12 the C 6 heavy ion irradiation (dose the 6 × 8 ions / cm 2 , 1.8 × 10 9 ions / cm 2 and 3.6 × 10 9 ions / cm 2 ) detailed observations of many biological traits of flax and agronomic traits, results showed that the germination conditions, capsule number per plant, yield per plant, oil content and so by the profound impact of the heavy ion irradiation. M1 generation flax, 6 × 10 8 ions / cm 2 irradiation can cause flax germination rate, to promote plant height, enhanced pollen viability. Irradiation processing flax seed weight and oil content increased to varying degrees, the higher the radiation dose, the greater the value of both are 3.6 × 9 ions / cm 2 irradiation dose of flax seed weight and oil content in the control group, respectively, compared to 16.5% and 19.9% ??higher In addition to to pollen morphological changes at this dose. Irradiated of flax DNA molecules also produce the effect of screening out 18 random primers amplified clear and stable, reproducible DNA fragments, polymorphic DNA fragments ratio was 52.5%. Compared with M1 generation, due to the M2 generation benne have partially repaired the damage due to heavy ion irradiation, most of the observed indicators trend occurred a change, while only a small part to maintain the same trend. M2 generation flax screening suspected mutant, wherein some mutants having a use value in the actual production. In this study, the 6 × 10 8 ions / cm 8 -3.6 × 10 9 ions / cm 2 < sup> 12 C 6 heavy ion irradiation case, more performance Huma Sheng long developmental stimulation, cause of the variation is not too big, not wide enough. However, flax crops should increase 12 C 6 heavy ion irradiation dose, in order to produce more variability to expand mutation spectrum.

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