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Study on the Uptake of ^99mTc-DTPA-DG by Tumor Cells after Chemotherapy and the Uptake of ^99mTc-DTPA-DG by the Nucleus of Lung Cancer Cells Calu-3

Author: LiangJie
Tutor: ChenYue
School: Luzhou Medical College
Course: Medical Imaging and Nuclear Medicine
Keywords: 99mTc-DTPA-DG Deoxyglucose Cell lines, tumor Radionuclide imaging Chemotherapy, adjuvant Radiopharmaceuticals Nucleus
CLC: R734.2
Type: Master's thesis
Year: 2008
Downloads: 35
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Abstract

Objective: ① explore 99mTc-labeled diethylenetriamine pentaacetic acid - deoxyglucose (99mTc-diethylenetriamine pentaacetic acid-deoxyglucose, 99mTc-DTPA-DG) monitoring the early effects of cancer chemotherapy. The false-positive rate, (2) explore whether 99mTc-DTPA-DG into the nuclei of tumor cells, thereby reducing tumor diagnosis to further verify the potential feasibility for tumor imaging. Methods: ① A549 lung cancer cells cultured in vitro (adenocarcinoma), MCF-7 breast cancer cells and colon cancer cells HT-29 added to 24-well plates, each divided into adding chemotherapy drug chemotherapy group and join the saline negative control group, selected chemotherapeutic drugs cisplatin (5,10 μg / ml), paclitaxel (7,14,28 μg / ml) and 5 - fluorouracil (5-fluorouracil, 5-Fu, 35,70 μg / ml) of. Chemotherapy drugs after 6h, the chemotherapy group and control group changes in cell morphology and the presence of apoptotic bodies was observed under an inverted microscope, digestion and the cells were collected, parallel phospholipid binding protein V (Annexin V, AV) / propidium iodide (propidium iodide, PI) double labeling staining, flow cytometry apoptosis rate of the cells in each group. Chemotherapy drug after 4h, the chemotherapy group and the control group cells were added 37MBq / L 99mTc-DTPA-DG 0.1ml / well and cultured for 2h, digestion and the cells were collected, γ measurement instrument detection rate of uptake of 99mTc-DTPA-DG . ② lung cancer cells cultured in vitro Calu-3 (adenocarcinoma) divided into five groups: a 99mTc-DTPA-DG group b. 18F-FDG group c. 99mTc-DTPA group, d. 99mTcO4-group, e. saline group. Set up in each of the three concentrations of each drug, and that every hole by adding one radiopharmaceutical 5,10 or 20μCi/0.1ml, adding an equal amount of saline control group, each group set up in each of the five wells. Various drugs and saline reaction 2h, digestion and the cells were collected, γ measuring instrument to detect the expression radioactive counts, osmotic swelling method separation nucleus, detection of the nucleus of radioactive counts isolated nuclei smear row hematoxylin eosin (hematoxyline eosin, HE) stain microscopy. Results: ① A549 lung cancer cells, MCF-7 breast cancer cells and colon cancer cells HT-29 were treated with cisplatin, paclitaxel, and 5-Fu role 6h after inverted under the microscope, appears obvious apoptotic morphological changes were seen in cells, and visible formation of apoptotic bodies. Flow cytometry showed the apoptosis peak and the higher rate of apoptosis. Chemotherapy group of three tumor cell apoptosis rate compared to the control group were significantly increased, and the differences were statistically significant (P lt; 0.05), and apoptosis rate gradually increased with the concentration of chemotherapeutic drugs. gamma measurements detected three tumor cell chemotherapy group 99mTc-DTPA-DG uptake was significantly lower than the control group (P lt; 0.05), with the concentration of chemotherapeutic drugs, 99mTc-DTPA-DG uptake rate gradually decreased. Examination see ② lung cancer cell line Calu-3 separation nucleus smear HE staining nuclei were hematoxylin stained blue-purple cytoplasm eosin stained pink, a small number of the latter count results showed that the nucleus count cells total count The proportion of 95%. gamma measurements detected lung cancer cells in Calu-3 of 99mTc-DTPA-DG and 18F-FDG uptake of radioactivity group was significantly higher than that of 99mTc-DTPA 99mTcO4-saline group, the difference was statistically significant (P lt ; 0.05), and lung cancer cells was significantly higher than that of 18F-FDG uptake of 99mTc-DTPA-DG uptake. 99mTc-DTPA-DG group with radioactivity increase in lung cancer cells gradually reduce their intake rates. 18F-FDG group with radioactivity increase in lung cancer cells than the first elevated to reduce their intake. Tumor cells compared to the uptake of 99mTc-DTPA and 99mTcO4-saline group difference was not statistically significant (P gt; 0.05). Nucleus count results show that 99mTc-DTPA-DG group enters the nucleus ratio was significantly higher than that of 18F-FDG group (P lt; 0.05). Conclusion: ① tumor cells by 99mTc-DTPA-DG uptake rate change reaction effect of chemotherapy, 99mTc-DTPA-DG decreased uptake, reflecting the effect of chemotherapy, and vice versa, said the effect of chemotherapy, it is a 99mTc-DTPA-DG could be used for tumor imaging agent for early assessment of the effect of chemotherapy. ② 99mTc-DTPA-DG can enter the nuclei of tumor cells, it is valuable to distinguish between inflammation and tumor, can reduce the false-positive rate of tumor diagnosis is a potential targeted molecular imaging agents for tumor imaging.

Study on the Uptake of 99mTc-DTPA-DG by Tumor Cells after Chemotherapy and the Uptake of 99mTc-DTPA-DG by the Nucleus of Lung Cancer Cells Calu-3

Abstract

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Study on the Uptake of ^99mTc-DTPA-DG by Tumor Cells after Chemotherapy and the Uptake of ^99mTc-DTPA-DG by the Nucleus of Lung Cancer Cells Calu-3