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Obesity is a chronic disease hazard to human health, is increasingly becoming a serious threat to the global epidemic. The World Health Organization (World Health Organization, WHO): With the social and economic prosperity, excessive caloric intake in the diet caused a certain degree of overweight and fat layer is too thick, body fat, especially triglycerides accumulate excessive lead to an obese state, developed and developing countries, the most common serious impact on human health of chronic non-communicable diseases. The treatment of obesity based on improving the way of life, increased physical activity, drug control or surgical treatment, but the effect is less than satisfactory, safe and effective weight-loss drugs and therefore seek to explore the mechanism of action of the drug become hotspot medical work. Betaine in animal husbandry as lean meat agent in feed use. Our laboratory research work has also been found that betaine intake of high-fat obese rats significantly inhibited heat, food intake and body weight, and serum malondialdehyde levels can reduce elevated antioxidant capacity, serum leptin reduce and IL-6 levels. Betaine on whether there is a direct impact at home and abroad isolated adipose tissue has not been reported. The experimental rats from adipose tissue, adding different drugs in incubation under certain nutritional environment and conditions, observed isolated adipose tissue release of free fatty acids (the Free fattyacid FFA), malondialdehyde (malondialdehyde MDA), total antioxidant capacity (total antioxidant capacity, T-AOC), and leptin (of leptin), tumor necrosis factor-a (tumor necrosis factor-a, TNF-a) and white interleukin -6 (interleukin-6, IL -6) impact and explore its possible mechanism. Healthy SD rats weighing 250 ± 20g. Experimental groups: normal control group: Add 0.9% saline; epinephrine hydrochloride group: 10 -6 mol / L epinephrine hydrochloride; drug group: including the Betaine low dose (10 -7 mol / L) group, betaine dose (10 -6 mol / L) in group, and betaine high dose (10 -5 mol / L) group; the mechanism group in dose betaine plus 10 -5 mol / L Inderal (propranolol in), verapamil given (varapamil) and adenylate cyclase inhibitor (SQ22, 536). The the experimental rats dislocated sacrificed retroperitoneal adipose tissue were quickly removed, weighed, each fat pad weight of approximately 50.0 ± 2.0mg, then cut into small pieces, placed in 37 ° C containing bovine serum albumin (0.04g/ml) 2ml Krebs solution incubated in the incubation mixture were added to different concentrations of betaine or blocker plus betaine, adding drugs capacity each 20μl. The adipose tissue was incubated in the continuous process to pass into 95% O 2 and a 5% CO 2 of the mixed gas, so that the pH value of the solution was maintained at 7.4. The specimens were incubated for 90min, and absorb the supernatant was measured FFA content of malondialdehyde (MDA), total antioxidant capacity (T-AOC) level the adipocytokines leptin (leptin), tumor necrosis factor - a (TNF-a) and white interleukin -6 (IL-6) content. All data is statistical software SPSS11.5 processing, experimental data were presented as mean ± standard deviation ((?) ± S) between groups compared by one-way ANOVA, P lt; 0.05 the difference was statistically significant . The experimental results are as follows: 1 betaine and blocker plus betaine isolated adipose tissue release FFA. Compared with the normal control group, epinephrine hydrochloride significantly promote the release of rat isolated adipose tissue FFA; betaine low dose group, no significant role in promoting the release of FFA; betaine middle dose group and high dose group could significantly promote the FFA released. And the betaine in dose group compared with propranolol to block β receptor, verapamil blocking L-type Ca 2 channels and inhibition of adenylate cyclase (adenylate cyclase, the AC) averaging section reduces the betaine to promote the release of FFA from adipose tissue. 2 betaine and blocker plus betaine vitro adipose tissue MDA and T-AOC levels. Compared with the normal control group, epinephrine hydrochloride significantly reduced the MDA, increased T-AOC; betaine dose group in levels of MDA decreased, increased T-AOC. Joined propranolol, verapamil and SQ 22,536 after co-incubation with betaine, betaine reduce malondialdehyde (MDA), elevated level of total antioxidant capacity can be partially suppressed. 3 betaine isolated adipose tissue release leptin, IL-6 and TNF-a levels. Compared with the normal control group, epinephrine hydrochloride adipose tissue release leptin, IL-6 and TNF-a level of no significant impact; while in the dose of betaine can significantly promote the release of adipose tissue leptin, but IL-6 and TNF-a had no significant impact. This experimental study showed that: betaine can significantly promote the release of rat isolated adipose tissue FFA improve the body's antioxidant capacity to promote the release of leptin. Its mechanism of action may be part of adrenaline through the fat cell membrane β receptor, and thus from the upstream of the protein kinase A (protein kinaseA, PKA) to promote the synthesis of cyclic adenosine monophosphate (cyclic adenosinemonophosphate, cAMP); probably through cell membrane of of Ca 2 channel open, causing calcium release and extracellular calcium influx, in turn activate PKC (protein kinase C, PKC), increased activity of hormone-sensitive lipase (hormone-sensitive lipase, HSL) achieved.
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