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Production of FSH Transgenic Pigs

Author: LiaoRongRong
Tutor: RenJun；HuangLuSheng
School: Jiangxi Agricultural University,
Course: Animal Genetic Breeding and Reproduction
Keywords: Pig Transgenic FSHα High fecundity
CLC: S828
Type: Master's thesis
Year: 2011
Downloads: 90
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Abstract


China is the world's first production of live pigs and pork consumption big country the pig industry occupies an important position in China's national economy . Reproductive traits is one of the important economic traits in the pig industry , including litter size , number born alive , conception rate . Reproductive traits of low heritability traits complex polygenic control , mainly influenced by the variety of genetic , environmental , nutritional , age, management and utilization . An important indicator to measure the level of reproductive traits litter size , the trait is mainly affected by the ovulation rate , conception rate , embryo survival rate and environmental effects of a variety of factors , the slow progress of conventional breeding . Since 1980 Gurdon for the first time obtained from the transgenic mice , breeding experts succession of transgenic technology used in livestock and poultry breeding , reproductive traits , including production performance expectations of rapid increase . Studies have shown that the follicle-stimulating hormone (FSH) gene is the major gene affecting litter size in pigs , the present study intends to adopt somatic cell cloning to produce transgenic FSH cloning pigs transgenic technology to improve pig farrowing performance . The the BAC vector of this study FSHα gene , defective λ prophage mediated homologous recombination pig FSHα-BAC Erhualian modified inserted Neo gene can produce anti- G418 protein , to obtain FSHα-loxp-Neo -loxp-BAC transfected gene vectors , gene vector is then obtained by the same method FSHss - the loxP - with Neo - the loxP - BAC turn mixed transfection Tai Landrace fetal fibroblast cells , stable expression of anti- G418 protein of transgenic positive cells by G418 by microinjection , somatic cells injected into enucleated porcine oocyte fusion electric shock at the same time to activate transgenic embryos and transferred to a chemically enhanced activation drops to handle 3 - 4h to be the the embryonic length to 2-4 cells after surgery Act transplanted into the the receptor sows tubal deep , waiting for the transgenic pig was born . BAC -mediated transgenic overcome the high copy number of exogenous gene integration , incomplete genetic elements ; Spindle-view the spindle showing nuclear Law enucleated operating system for microscopic high enucleation rate . In this study, laid an important preliminary work for the final preparation of a transferred the FSH gene cloning pigs with high reproductive potential .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Pig
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