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Study on the Salt Stress Adaptaion of Leguminous Plant Alhagi Pseudoalhagi
Author: WuMuTiHan
Tutor: HaiLiLi·KuErBan
School: Xinjiang Agricultural University
Course: Zoology
Keywords: Salt stress Photosynthesis Antioxidant enzyme system Legume Alhagi pseudalhagi Green beans
CLC: S793.9
Type: Master's thesis
Year: 2011
Downloads: 85
Quote: 1
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Abstract
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The Salt Stress is serious affect plant growth and cause crop failures abiotic factors. To this end, increase plant yield, and not just rely on to increase their genetic potential, and more importantly, should focus on the study found that nature existing strong resistance, high salt, drought, soil fertility barren environment to maintain effective yield excellent species Alhagi (Alhagi pseudalhagi Desv.) is growing in desert and semi-desert area of ??perennial legumes in Tarim Basin Alhagi is both an important natural sand-fixing plant species, herbivore indispensable quality forage one. The experiment the legumes Alhagi with mung bean (Vigna radiata) do control, A Comparative Study of the two types of plant salt tolerance analysis of photosynthetic carbon assimilation. Four concentrations of the plant material grown in the greenhouse plastic pots containing vermiculite with 1/2 hoagland culture medium, set 0 (control), 50, 100, and 200 mM NaCl treatment two weeks measured photosynthesis-related parameters. Different response to salt stress salt-tolerant plants Alhagi with mung bean photosynthetic capacity of salt-sensitive plants. The mung bean Pn \u0026 value decreases with increasing salt concentration Ci value is unchanged. Sparsifolia 50 mM treatment photosynthesis was significantly higher than the control, and reached 130%, 100 mM of processing and controls did not differ significantly when reduced to 60% of the control to 200 mM. The mild under salt stress Pn increased with gs low is closely related to the Ci value slight drop CO2 assimilation rate enhancements led to the rise of Pn. In addition, the activity of Alhagi Rubisco protein content of the enzyme in different salt treated salt concentration increased when 50mM Pn Rubisco synthesis rate and activity increase caused: 100 mM treatment Rubisco and content of the highest activity relatively low, so that the CO2 assimilation rate and the control there was no significant difference in: 200 mM processing enzyme under the synthesis and activity at the same time is restricted. Salt sensitive plants mung bean photosynthesis decline in the low-salt stress is restricted because the electron transfer rate PS Ⅱ (ETR) and PS Ⅱ Fv / Fm decline, consistent with previously reported. NPQ significantly increased the form of heat scattering showed that the majority of electronics to reduce the electron transfer rate, to avoid harm caused electron accumulation. Salt-tolerant plants Alhagi pseudalhagi the CO2 assimilation efficiency, promote low salt stress can promote Φps Ⅱ to determine, ETR also elevated pressure due Φps Ⅱ. Fv / Fm caused by elevated ΦPS Ⅱ and qP increased because the rate of synthesis of low salt concentration or antenna pigment complex induced PS II complex. The total chlorophyll content in mung bean salt treatment significantly reduced Chla / b value increased. Alhagi treated total chlorophyll and chlorophyll b content was significantly high Chla / b values ??decreased significantly. Alhagi Chla / b lower value that the PS Ⅱ / PSI changes caused the. Thus, the salt treatment after mung chlorophyll content is reduced because of lower psi composite of magnitude higher than PS Ⅱ. So as to arrive Alhagi photosynthesis can be sure that the response to salt stress due to Rubisco synthesis rate and the enhanced activity of the PS complex. Salt stress, especially at high concentrations (100 and 200mmM) under salt stress taproot and leaves of salt-tolerant plants Alhagi SOD activity was significantly higher than the untreated control, because the root is the first organ by stress, Alhagi taproot O2-clear synthetic the highest H2O2 ability, accompanied POD activity significantly induced rise in various organs, and also has strong decomposition of H202. Mung bean POD activity decreased. Fibrous roots of SOD activity, mung bean induced strong induction Alhagi POD activity decreased, this may be the mung bean fibrous roots due to a reduced ability of H202. Therefore, Alhagi may rely mainly on POD Clear H2O2 - against oxidative stress. Alhagi leaves and stems of CAT activity increased significantly, but the activity is not apparent in the mung bean. Of Alhagi in SOD salt-induced upregulation of POD and CAT activities, Alhagi than mung bean has the ability to more effectively clear H202, this may be one of the reasons Alhagi with strong salt resistance.
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