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The Study of the Antibacterial Activity Components and the Polysaccharides of the Dictyophora Echinovolvata Volva

Author: LinChenQiang
Tutor: LinXinJian
School: Fujian Agriculture and Forestry University
Course: Microbiology
Keywords: Spine Dictyophora Natural Products Biological activity Development and utilization
CLC: S646.8
Type: Master's thesis
Year: 2011
Downloads: 46
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Abstract


The papers cited as experimental subjects, the self- the Fujian Shunchang mushroom Office spine of Dictyophora (Dictyophora echinovolvata) , is divided into three parts : the edible parts of the bacteria ( stipe and bacteria - skirt ) , cap ( spore body ) attached to the cap on and volva ( outer membrane , the membrane between glial ) , this paper studies the nutritional value of the volva , volva in antibacterial substances and polysaccharides , as Dictyophora volva development utilize to provide a scientific basis . The results are as follows : (1) The test results show that : the spines of Dictyophora the volva accounts fruiting bodies of fresh weight of 56% , the cap 11% , only 33% of bacteria . The volva of nutrients and cell close to the value of development and utilization . ( 2) the use of different polarity reagents extract the volva antibacterial substance : volva sifted through a 40 mesh sieve methanol extract 48h best results ; to different bacterial indicator bacteria , determination the volva Dictyophora ethyl acetate antibacterial activity of the crude extract , the results showed that : volva Dictyophora ethyl acetate extract inhibit the use of several types of bacteria are indicator bacteria Staphylococcus aureus , antibacterial substances has a better stability of temperature , ultraviolet sex . (3) The bamboo fungus volva crude extracts were separated by silica gel column , 3 antibacterial activity component DEV I DEV Ⅱ , DEV Ⅲ higher activity the DEV Ⅰ by the secondary silica gel column chromatography , a bacteriostatic the role of the component number 7. Scanned by UV spectroscopy and its components at 244nm and 274nm at one each absorption peak area of ??nearly two absorption peaks , still can not determine its principal component . DEV Ⅲ subjected to reverse phase column chromatography , to obtain two kinds of bacteriostatic component DEV Ⅲ -1 and DEV III-2 , and DEV III-2 by the second reverse phase column chromatography to obtain the bacteriostatic component DEV III -2-2 DEV Ⅲ -1 and DEV Ⅲ -2-2 spectral scanning , both in the two main absorption peak wavelength of 210nm and 330nm . ( 4) orthogonal test of volva Dictyophora polysaccharide optimum extraction process conditions : volva broken after 40 mesh sieve material to water ratio of 1:30, extraction temperature 90 ° C , extraction 3h, polysaccharide extraction rate 1.356 g/100g. The crude polysaccharide after cellulose column separation and purification , get the three crude polysaccharide DEVP1, DEVP2, DEVP3, DEVP1 DEAE SepuaroseTM Fast Flow column purification Polysaccharide DEVPa purity identification of the ultraviolet spectrum , to show that DEVPa for single polysaccharide fraction .

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CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Fungi ( mushroom ) > Dictyophora
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