Dissertation > Excellent graduate degree dissertation topics show

Establishment of Technique System of Agrobacterium-mediated Transformation Cotton (Gossypium Barbadense L.) and Identification of Resistance to Cotton Boll Worm of Bt Transgenic Cotton

Author: ZhouLiRong
Tutor: QuYanYing；ChenQuanJia
School: Xinjiang Agricultural University
Course: Crop Genetics and Breeding
Keywords: land cotton island cotton regeneration system differences Bt genetic transformation
CLC: S562
Type: Master's thesis
Year: 2011
Downloads: 47
Quote: 0
Read: Download Dissertation

Abstract


In this study, we studied the difference on regeneration by somatic of land cotton(simian3,kezimian312) and island cotton (xinhai30,xinhail6) and finded the difference of culture prosess on the two cultivars, We maked hereditary transform of Bacillus thuringiensis toxin protein gene (Bt) by Agrobacterium.In order to conform foreign gene have integrate into genome by PCR on T1 and RT-PCR on T2. We reguired transformatic plantet and Identfied the resistance worm to requiring transformationg planet. The results of worm feeding intial certificate this.The results of this research such as:1.We used hycopotys of island cotton and land cotton as explant.We used Screening the best condition of embryo callus induction condition under the same methed of callus induction and proliferation. The best embryo callus induction mediun on land cotton is MSB adding 1.9 g/L KNO3, The rate of embryo callus induction reach 70%. The best embryo callus mediun on island cotton is MSB adding 1.9 g/L KNO3 and 0.3mg/L NAA. The best embryo callus multiplication mediun on land cotton is MSB adding 1.9 g/L KNO3, 0.1 mg/L Glu and 0.1mg/L Asn. The best embryo callus multiplication mediun on island cotton is MSB adding 3.8 g/L KNO3,0.1 mg/L Glu and 0.1 mg/L Asn.2. The best embryo Somatic embry differentiation mediun on island cotton is MSB adding 1.9 g/L KNO3, 0.1 mg/L Glu,0.1 mg/L Asn and 0.05m/L IBA.3. Arobacterium-mediated embryo callus of island cotton xinhai30 and xinhai 16, Requiring BT gene transgenic plants. Converting conditions for:Island cotton embryo callus was soaked bacterium fluid with OD of the value of 0.5～0.6,15min. post-communist 24h. Resistent embryo callus will turn into adding 50mg/L kana and 400mg/L cephalosporins amphotericin medium induced somatic embryos genetically differentiation. The best seedlings medium of resistance cotyledons embryo is 1/2MSB adding 300mg mg/ L cephalosporins amphotericin.4. We screened the kanamycin resistence on the transgenic plants of T1 generation.The results shows that resistance rate is 12.6%. Detection by PCR detection on T1 transgenic plants of positive kanamycin resistence Then identify the insect resistance of T1 generation transgenic plants, insect resistance results show that transgenic island have insect resistance. We got T2 generation the transgenic plants tissue seedlings by tissue culture of T1 generation of transgenic island cotton. Identify T2 generation of the transgenic plants by RT-PCR detection, the results showed that Bt could be normal expression in transgenic T2 plants.

Related Dissertations

  1. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  2. Transformation of RdreBlBI Gene Drived by Rd29A in ’Benihope’ Strawberry,S668.4
  3. Soil Eoclogical Risk Evaluation of Transgenic Bt Rice Through Nematode Community Analysis,S154.1
  4. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  5. Fitness Costs of a Disrupted Cadherin Allele Conferring Cry1Ac Resistance of Helicoverpa Armigera,S435.622.3
  6. Studies on the Differences of Tolerance between Chilo Supperssalis Walker and Sesamia Inferens Walker to Bt Rice,S435.112.1
  7. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  8. Effects of Nitrogen Management Practices on Expression and Degradation of Bt-protein in Transgenic Cotton,S562
  9. The Genetic Transformation and Functional Analysis of GmFtsH,S565.1
  10. Cloning of Proteinase Inhibitor Gene CYS and API from Wild Potatoes and Preliminary Study of Potato and Tobacco Transformation with Spcys,S532
  11. Construction of Expression Vector and Genetic Transformation in Phalaenopsis of Phalaenopsis Floral Meristem Identity Gene LFY,S682.31
  12. Study on Transformation of Cut Chrysanthmemum with DdICE1 Gene,S682.11
  13. Study on Agrobacterium-Mediated Transformation of ’Benihonpe’ Strawberry with ALA Synthase Gene,S668.4
  14. Preliminary Study on the Regeneration System and the Reporter Gene GFP Instantaneous Expression of Soft-seed Pomegranate Variety ’Tunisia’,S665.4
  15. Functional Analysis of Solanum Torvum StPGIP Gene Related to Verticillium Resistance,S572
  16. Transformation of Tobacco with Arabidopsis Thaliana MAP65-6 and TUA-6,Q943.2
  17. Transgenic Maize Plants Obtained by Pollen-mediated Transformation and Agronomic Trait Investigation of Transgenic Maize Hybrids,S513
  18. Study on Novel Germplasm of Flowering Controllable Chrysanthemum,S682.11
  19. Endosperm-specific Expression Vector Construction and Genetic Transformation of Wheat Avenin-like b Gene,S512.1
  20. The Isolation and Genetic Transformation of TaCBL3 in Wheat (Triticum Aestium L.),S512.1
  21. Construction of RNA Interference Vectors of Pin Genes Determining Grain Hardness and Genetic Transformation in Wheat,S512.1

CLC: > Agricultural Sciences > Crop > Economic crops > Fiber crops > Cotton
© 2012 www.DissertationTopic.Net  Mobile