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Broad-spectrum Resistance Gene Engineering Research Based on the Interaction of R Gene and Cognate Avirulence Gene

Author: ZhangHongXia
Tutor: DengQiYun；ZhouBo
School: Central South University
Course: Crop Genetics and Breeding
Keywords: M. grisea Dual expression vector Differentially expressed Induced expression of the promoter Optimized system
CLC: S511
Type: Master's thesis
Year: 2011
Downloads: 118
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Abstract

High and stable yield of rice directly related to national food security and social stability. Magnaporthe harm caused by the drop in grain production has become one of the main reasons for the slow growth in China's grain output. Spraying pesticides to control diseases cause significant pollution to the environment, but also ineffective. 3-5 years after the promotion in the most resistant varieties in production lead to resistance to the loss of new physiological races because the pathogen variation. Cultivate durable resistant varieties to control rice blast disease resistance breeding strategies need to innovate. The specific interaction between the rice and rice blast fungus in line with the gene to gene (gene-for-gene) 亻 paragraph says. According to this hypothesis, de Wit proposed plant factor broad-spectrum resistance strategy: upcoming R gene and the corresponding Avr gene on the to import host plant pathogens invade the promoter of the inducible expression host infection by bacteria, Avr gene were induced to express the the AVR proteins stimulate its specific R gene expression to produce the corresponding receptor protein, mutual identification between them, resulting in the HR response of the host, to prevent pathogen colonization and extension of the invasion, while also further induced systemic acquired resistance (systemic acquired resistance, SAR), the plant broad spectrum resistance to multiple pathogens. The key is the interaction of R / Avr gene pairs, and can only be triggered by pathogen infection appropriate promoter. This study is based on genetic engineering of this broad-spectrum disease resistance breeding strategy, screened by the rice blast fungus invasion induced specific expression promoter, the interaction of rice blast resistance gene Piz-t, and rice blast fungus avirulence gene the Avr-pizt set this promoter to construct the dual expression vector. Built based on this study, transgenic receptor rice varieties Agrobacterium mediated the rice transgenic optimization system, expectations induced expression of specific genes into rice, broad-spectrum disease resistance breeding to improve the resistance of rice. This study achieved the following results: 1 previous 23 promoter of the human study results based on further screening analysis, selected nine of them as a candidate promoter and cloning. These nine promoter rice blast infection 0h to 96h expression analysis, found that the expression of genes Loc ₀ 4g10160 the rendering characteristics of \specific promoter; 2 were constructed to complete a total of 27 expression vector of the three kinds of the carrier 9 promoter, including nine candidate promoter replaces the CaMV35S promoter pCambia 1305.2 start GUS gene and construct pCSPIRB (pCambia vector for Screen Promoter Induced by the Rice Blast Invasion) vector; the the candidate promoter and Piz-t, the avirulence gene AvrPiz-t, and rice blast resistance gene pCDRRB 1 (pCambia vector for Durable Resistance to Rice Blast) series carrier; candidate promoter The avirulence gene AvrPiz-t90 and rice blast resistance gene Piz-t pCDRRB2 series carrier. 3 by setting different media, hormone combinations Agrobacterium dip experimental treatment time, rice mediated by receptors varieties Y58S transgenic optimize the system, to build dual expression vector efficient transfer to the rice.

Broad-spectrum Resistance Gene Engineering Research Based on the Interaction of R Gene and Cognate Avirulence Gene

Abstract

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