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Preparation and Application of Bonded Amylose and Amylopectin Chiral Stationary Phases for Separation of Enantiomers

Author: ChangJing
Tutor: HanXiaoZuo
School: Lanzhou Jiaotong University
Course: Chemical processes
Keywords: High performance liquid chromatography chiral separation bonded chiral stationary phase
CLC: O657.72
Type: Master's thesis
Year: 2009
Downloads: 168
Quote: 1
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Abstract


With the development of science and technology, the importance of chirality has constantly increased in the last decades, because the enantiomers of the drug often display different biological activities in pharmacological and metabolic properties. Increasing demands for enantionmerically pure compamds have resulted in chiral separation becoming one of the most important analytical tasks. Chiral separation of enantiomers using high performance liquid chromatography becomes one of the most powerful and widely employed techniques, especially directly chiral separation using chiral stationary phase.In this thesis, four bonded amylose and application chiral stationary phases were prepared and characterized by IR sepctroscopy. These CSPs were used to study the chiral separation of some chiral compands.This thesis is composed of five chapters:Chapter one reviewed the importance and methods of chiral separation of chiral compounds, It is given emphasis on the development and types of chiral stationary phases by high performance liquid chromatography.In chapter two, three, four, five, bonded amylose-（3,5-dimethylphenylcarbamate） chiral stationary phase, amylose-（phenylcarbamates）chiral stationary phase, amylose -（Cl-phenylcarbamates）chiral stationary phase, amylopectin-（3,5-dimethylphenylcarbamate） chiral stationary phase were prepared and characterized with IR sepctroscopy. The effect on separation of modifiers （dichloromethane, trichloromethane and Tetrahydrofuran） were investigated. It was found that these modifiers can affect the separation; and enantiomer separations were improved to different extents by THF, CH2Cl2, CHCl3 added to mobile phase. The results revealed that the bonded CSPs exhibited a varying chiral recognition ability for chiral compounds in this study.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Chromatographic analysis > Liquid chromatography analysis
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