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The Effects of Sufentanil Post-conditioning on hepatocellular Apoptosis Induced by Intestinal Ischemia-reperfusion in Rats

Author: ZhaoLi
Tutor: CaoDingZuo
School: Shanxi Medical
Course: Anesthesiology
Keywords: Sufentanil After-treatment Ischemia-reperfusion injury Liver cells Apoptosis
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 1
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Abstract


Objective To investigate the post-processing of Sufentanil (sufentanil post-conditioning) on ??intestinal ischemia reperfusion liver cell apoptosis and apoptosis-related proteins Bcl-2 and Bax expression in liver tissue impact. 40 method healthy adult Wistar rats, male and female, regardless of weight about 200g-250g, were randomly divided into five groups (n = 8): ① the sham group (S): This group only line the middle of open threading without folder closed the superior mesenteric artery, more than the same intestinal ischemia-reperfusion group. ② intestinal ischemia-reperfusion (IIR group): occlusion of the superior mesenteric artery, superior mesenteric artery ischemia last 5 minutes were given saline, the process of post-processing with sufentanil group. ③ sufentanil deal with low-dose group (SP1 group). The ④ sufentanil processing middle dose group (SP2 group). ⑤ sufentanil after processing the high-dose group (SP3 group). SP1-3 group in the superior mesenteric artery ischemia via the femoral vein in the final five minutes were given of sufentanil 1μg/kg, 5μg/kg and 10μg/kg configuration into 0.5 ml of the liquid, with a tiny syringe pump in five minutes pumped into I with IIR group. S group, IIR group pump into 0.5ml of saline. Intestinal ischemia-reperfusion liver injury model folder by using the minimally invasive arterial occlusion of the superior mesenteric artery preparation. Reperfusion rats were sacrificed at 3h, based on the need to deal with the left lobe of the liver tissue, light microscope liver pathology, immunohistochemistry and medical image analysis system in the liver tissue of Bcl-2 and Bax protein semi-quantitative analysis, and using terminal deoxy nucleotide transferase-mediated dUTP in situ nick end labeling (TUNEL) technology to detect and calculate the hepatocyte apoptosis index (AI). Results ① optical microscope: SP rats was significantly lower than the degree of liver injury IIR group. ② The SP group hepatocyte apoptosis index (AI) was significantly lower than the IIR group (P lt; 0.05). (3) each SP group Bcl-2 protein expression mean optical density (OD) value was significantly higher than the IIR group (P lt; 0.05), while the average optical density (OD) value Bax protein expression was significantly lower than the IIR group (P lt; 0.05). Compare each index difference between ④ SP group group was not statistically significant (P gt; 0.05). Conclusion ① Sufentanil post-processing can reduce liver injury induced by intestinal ischemia-reperfusion. ② sufentanil three different concentrations, no significant difference in treatment to reduce the damage to the liver tissue. The ③ sufentanil after processing to reduce liver injury induced by intestinal ischemia-reperfusion, the mechanism may be with upregulation of Bcl-2 expression and inhibit Bax upregulated related.

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