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Immunofluorescence Study of LPS-induced NF-κB Expression in Human Decidual Cells

Author: Camara Mamoudou(MaMuDu)
Tutor: YaoRuoJin
School: Central South University
Course: Gynaecology
Keywords: Nuclear factor kappa B Decidual cells Lipopolysaccharide
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
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Abstract


Objective: The purpose of this study is to explore the different doses of lipopolysaccharide -induced early pregnancy human decidua nuclear factor κB (NF-κB) expression . METHODS: Human decidual cells taken from 10 healthy early pregnancy patients induced abortion , isolated decidual cells treated without dose of lipopolysaccharide (LPS) , immunofluorescence staining of nuclear factor KB protein expression differences . Results: The results between the different experimental groups statistically significant difference ( P ≤ 0.05 ) . The use of single-factor analysis of variance and Dunnett's T3 test , all data mean ± standard error expression . Conclusion: This study showed that lipopolysaccharide (LPS) and human decidual activation of nuclear factor KB signal transduction pathways , the higher the concentration of fat more than the stronger expression of nuclear factor -KB .

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