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The Effects and Mechanisms of Infliximab on Rat Colitis Induced by Trinitrobenzene Sulfonic Acid

Author: LiuXianMin
Tutor: MouFangHong
School: Liaoning Medical
Course: Internal Medicine
Keywords: Ulcerative Colitis Inflammatory bowel disease Trinitrobenzene sulfonic acid Infliximab Cytokines
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 33
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Abstract

Objective To investigate the therapeutic effect of infliximab trinitrobenzene sulfonic acid induced colitis, and to study its mechanism of action, and to provide an experimental basis for the clinical treatment of ulcerative colitis. Methods 20 healthy male Wistar rats were randomly divided into normal control group and model group, n = 10. Model group using 5% trinitrobenzene sulfonic acid / ethanol solution (100mg/kg /) enema induced colitis model, normal control group isovolumic saline enema. Both groups 7 days after the model was given 0.9% sodium chloride 2.0ml orally, once daily for 14 days. 21 days, all rats were sacrificed and specimens were taken, rats, disease activity index, colon gross morphological injury indices and histopathological damage index score, decision modeling success. Selected on the basis of this experiment 40 rats were randomly divided into normal control group, model group, prednisone group and the infliximab group (n = 10). In addition to the normal control group, the remaining three groups making use of such methods colitis model. Given infliximab 30mg/kg infliximab group from the first 7 days 7 and 14 days, respectively, administration, intraperitoneal injection; prednisone group was treated with prednisone acetate 6mg/kg normal control group and model The group were given dissolved 0.9% sodium chloride, both orally once a day for 14 days. 21 days, all rats were sacrificed and specimens were taken, in addition to the score of the three indices, peripheral serum of the rats in each group, the colonic mucosa and spleen, tumor necrosis factor-α by enzyme-linked immunosorbent assay (ELISA). (tumor necrosis factor-α, TNF-α), interleukin (IL) -10 (Interleukin-10, IL-10) levels. Results Compared with the model group, normal control group rats disease activity index, gross morphologic damage index and histological damage index score was significantly lower, the difference was statistically significant (P lt; 0.01), trinitrobenzene sulfonic acid the enema can successfully induced colitis in rats. Infliximab group gross morphological damage index score compared with the model group was significantly decreased (P lt; 0.01), the other two rated lower (P lt; 0.05); prednisolone group compared with the model group in addition to gross morphological damage index score was significantly decline (P lt; 0.01), the other two scores decreased significantly (P gt; 0.05). Description infliximab has a good effect on rat colitis. Compared with the normal control group, compared with the model group, infliximab group peripheral serum model rats, colon mucosa and spleen of TNF-α were significantly increased, the difference was statistically significant (P lt; 0.01); content of TNF-α in the three tissues of rats, a statistically significant difference (P lt; 0.01); prednisone group compared with the model group, except weeks serum TNF-α levels were significantly decreased (P lt ; 0.01), the other two organizations had no significantly lower (P gt; 0.05). Compared with the normal control group, model group peripheral serum IL-10 was significantly decreased colonic mucosa, the difference was statistically significant (P lt; 0.01); infliximab group compared with the model group, peripheral Seiwa IL-10 content in the colonic mucosa was significantly higher (P lt; 0.01); prednisone group compared with the model group elevated IL-10 levels in the colonic mucosa (P lt; 0.05), but no significant peripheral serum l high (P gt; 0.05). Each group compared to the spleen of IL-10 content difference had no statistical significance (P gt; 0.05). Infliximab is possible to reduce the content of TNF-α in the body tissue, to improve the content of the IL-10, to thereby control the progress of inflammation, and prednisone acetate have a good effect on rat colitis. The conclusion given Wistar rats 100mg/kg / 5% trinitrobenzene sulfonic acid / anhydrous ethanol solution disposable enema can be successfully induced experimental colitis model. Infliximab can reduce the levels of TNF-α in the organization, to improve the content of IL-10, and progress in the control of inflammation, alleviate clinical symptoms of colitis in rats for the clinical treatment of ulcerative colitis certain experiments basis.

The Effects and Mechanisms of Infliximab on Rat Colitis Induced by Trinitrobenzene Sulfonic Acid

Abstract

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