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Changes in Endometrial Receptivity in Mice Based on Different Conditions

Author: SunXiaoJing
Tutor: ShiHong
School: Dalian Medical University
Course: Obstetrics and Gynaecology
Keywords: Endometrial receptivity Integrin Interleukin Ovulation induction drugs Semen
CLC: R714
Type: Master's thesis
Year: 2009
Downloads: 121
Quote: 2
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Abstract


Objective: In this study, mice study design 5 designs ovulation induction drugs, semen, seminal plasma analysis by measuring the expression of Wai window period mice endometrial integrin β3 subunit and interleukin-2 ,6 the impact of the embryo different factors on mouse endometrial receptivity. Methods: Female mice were divided into the pregnancy group and false pregnancy group, pregnancy group (divided into conception side of unilateral tubal ligation group, the nonpregnant side); false pregnancy group was divided into: natural cycle group, promote ovulation group and double side tubal ligation group. Each group at 2-7 days of gestation the uterine specimens were divided into 2 parts, -80 ℃ refrigerator. A line of frozen sections SP immunohistochemistry method determination of the expression of integrin β3; another line enzyme-linked immunosorbent assay (ELISA) method for the determination of the content of IL-2, 6. Results: 1. Integrin β3 Wai window period mouse uterus was localized in the cytoplasm of mouse endometrial glandular epithelial and luminal epithelial cells. Integrin β3 in the natural cycle of each group (single-bar group, the natural cycle group, double-bar group) mouse endometrium in pregnant 4d Table peaked expression, the peak reached in the group of ovulation pregnant 6d. In the the pregnancy group's expression in pregnant 4-5d than the expression of false pregnancy group (P lt; 0.05); expression in the natural cycle group, pregnant 4-5d than ovulation group (P lt; 0.05); natural cycle group and double-bar group expression in pregnant 2-7d were no differences (P gt; 0.05); single bar groups on both sides of the expression in the pregnant 2-7d and no differences (P gt; 0.05). 2. Interleukin -2 (IL-2): IL-2 in the mouse endometrium Wai window period regularly expressed. Ovulation in pregnant 5d expression level of the lowest, the rest of the group in pregnant 4d reached the lowest. Expression in the pregnant group in pregnant 3-4d below the false pregnancy group (P lt; 0.05). In the expression of the natural cycle group, in pregnant 3-4d below ovulation group (P lt; 0.05); the double bar group in 3-4d below the ovulation pregnant group (P lt; 0.05) natural cycle group and double bar group compared to 2-7d were no differences (P gt; 0.05) of pregnancy. Single bar groups on both sides of the expression in the pregnant 2-7d and no differences (P gt; 0.05). 3. Interleukin -6 (IL-6): IL-6 in the mouse endometrium Wai window period regularly expressed. Ovulation induction group reached the peak of pregnant 5d, the rest of the group reached a peak in pregnant 4d. The level of expression in the endometrium of pregnant mice at gestational 3-6d than false pregnancy group (P lt; 0.05). In the group expression ovulation, in pregnant 3-7d is lower than the other group (P lt; 0.05). In the expression of the natural cycle group, in pregnant 4-5d higher than the double-bundle group (P lt; 0.05). Single bar groups on both sides of the expression in the pregnant 2-7d and no differences (P gt; 0.05). Interleukin-2 ,6 expression in mouse endometrium Wai window period was a negative correlation (r = -0.78, P = 0.001). Conclusions: 1. Integrin β3-specific expression in the mouse endometrial epithelial and luminal epithelial cell cytoplasm, and consistent with the opening and closing of the window period, can be used as markers of endometrial receptivity. On endometrial receptivity may have a role in promoting. 2. Interleukin-2 ,6 expressed in mouse endometrium window period are peak was negatively correlated with each other mutual inhibition, to maintain a certain balance. Both involved in the establishment of endometrial receptivity, mechanism of action pending further study. 3. Compared with the natural cycle in mice, the application of gonadotropin ovulation induction, mice endometrial receptivity drop open window period delay. 4. Seminal plasma of mice endometrial receptivity may have a role in promoting; embryos by indirect role for mouse endometrial receptivity.

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