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Associativity Study between h-CD Degradation Regularity and the Estimation of Postmortem Interval in Stomach and Appendix of Mice

Author: LiuJia
Tutor: JiaoYan
School: Shanxi Medical
Course: Forensic
Keywords: forensic pathology highmolecular-weight caldermon(h-CD) postmortem intervals(PMI) western-blot
CLC: D919
Type: Master's thesis
Year: 2011
Downloads: 11
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Abstract


Objective：According to observe the postmortem degradation of high molecular-weightcaldesmon（h-CD）in stomach and appendix smooth muscle cells ofmice by Western Bloting，study the erects ofpostmortem intervals，and ambient temperature on the h-CD degradation ofmice，and the variability of the h-CD degradation in stomach smooth muscle cells and appendixsmooth muscle cells in different ambient temperature．Evaluate the application for estimatingthe time ofdeath in forensic medicine aspect．Methods：72 healthy adult Kunming mice were killed by asphyxia，and put them in 4℃refrigerator and 25℃artificial climate incubator respectively，then extract the stomach andappendix tissues at Oh，24h,48k72h，96h，120h after dead．The h-CD contents in the abovetissues were quamtitated by western-blot assay and collected by Image Pro Plus 5．0 imageanalysis system，then the datas were statistically analyzed with the SPSS 16．0 soflwaxe．Results：（1）After death，H-CD content in stomach and appendix tissues decreased with thepostmortem intervals，there is correlation between the postmortem degradation of h-CD and thepostmortem intervals;（2）The differences of the postmortem degradation of h-CD in different ambienttemperature were significant(P<0．05、．the degradation in 25℃faster than 4℃．The multipleregression equation between actin content and ambient temperature，PMl were：at 4C stomachtissues Y=14547．324-2574．543X，R2=0．997．appendix tissues Y=13326．062-2587．111X，R2=0．963;At 25℃stomach tissues Y=13917．886-2878．454X，R2=0．962．appendix tissuesY=11939．029-2663．071X，R2=0．870（Y was the IOD value of the h-CD content．X was thePMI，）（3）The differences of the postmortem degradation of h-CD between stomach andappendix tissues were significant（P<0．05），the degradation in the appendix more faster．ConelusionH-CD content in stomach and appendix tissues decreased gradually with the postmortem intervals，the postmortem degradation ofh-CD was influenced by ambient temperature，and thedegradation rate tended to be raised with higher temperature，and the differences of thepostmortem degradation of h-CD between stomach and appendix tissues were significant，thedegradation in the appendix more faster．The above analysis may be used for the estimation ofpostmortem intervals in forensic practice．

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