Dissertation > Excellent graduate degree dissertation topics show

The Experimental Study of MMP-2, MMP-9 and α2M in Severe Acute Pancreatitis

Author: ZhangYingYi
Tutor: WangZhongYu
School: Dalian Medical University
Course: General Surgery
Keywords: Severe acute pancreatitis Matrix metalloproteinase-2 Matrix metalloproteinase-9 α2-macroglobulins
CLC: R657.51
Type: Master's thesis
Year: 2009
Downloads: 36
Quote: 0
Read: Download Dissertation

Abstract


Severe acute pancreatitis is a hazardous disease which sometimes extends rapidly and occurs in 30 of one million people a year.It remains a high level of complications and mortality rate until today.A number of theories about the mechanisms and pathologic changes of SAP have been found in recent years and according to these theories the treatment and prognosis have changed gradually.The clinicians today have pay much more attention to the severity prospection and the extent prevention of SAP.α2M is a major serum protein with diverse functions,including inhibition of protease activity and disease factors.The matrix metalloproteinases are a family of peptidase enzymes responsible for the degration of extracellular matrix components including collagen,gelatin,fibronectin,laminin and proteoglycan.MMP-2 and MMP-9 mainly clear collagen typeⅣ,Ⅴwhich exist in basal memberane of endothelial cell.MMP-2 and MMP-9 are detected absence in most normal conditions contrast to a high presence in pancreatitis.As neutrophils of the inflammatory archetectures express MMPs and many cytokins stimulate the proliferation of nutrophils thus many of these cytokins can facilitate the release of MMPs. MMP-2 and MMP-9 play a important roll in the process of tissue necrosis ,neutrophil transendothelial migration ,circulation disorder and concrescence.In SAP ,considerable correlations about the imbalance of MMP-2,9 and their inhibitor--α2M in the pathologic mechanisms have been detected. MMPs and the inhibitor of them are becoming a new target in the process of therapeutic SAP.Our test is to investigate the effects of MMP-2,9 andα2M in the disorder of microcirculation on SAP and to show another way to prognose or to lessen the pathophysiological process of SAP.Objective To study the expression of MMP-2,9 in pancrease andα2M in serum in rats with SAP, compare the changed feature of pancrease on SAP and the correlation between them.Methods (1) To establish a SAP rat model then observe the wet weight and the pathochange of pancrease. (2) After it is confirmed to establish the SAP model, immunohistochemistry, spectrophotometer were used to detect the expression of MMP-2,9 and ELISA were used to detect the concentra- tion ofα2M in serum.Results 1.Through microscope ,cellular infiltration and necrosis were seen in SAP group. 2. Immunohistochemistry shows: There were highly expressions of MMP-2,9 in SAP group contrast with controls. It cxpressed 100% , 10% in SAP group and controls.Two groups had significantly difference. 3. ELISA: Compared with the control group, the concentration ofα2M in serum of SAP group increased respectively.Conclusions 1.Expressions of MMP-2 and MMP-9 have close relations with the injury of pancrease in SAP, so hasα2M. 2.The detection ofα2M combined with MMP-2 and MMP-9 might be a valuable way in serious prognosis and treatment in SAP.

Related Dissertations

  1. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  2. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  3. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  4. Effect of Acupuncture and Minimally Invasive Hematoma Aspiration on Rabbit Brain Damnification in Acute Stage of Intracerebral Hemorrhage,R245
  5. Studies on the Growth and Transfer Behaviour of Hca-F Cell and Immunomodulatory of fucoidan,R285.5
  6. The Changes of Intestinal Barrier Functional in Rats with SAP and the Effection of QingYi Ⅱ,R657.51
  7. α-Lipoid Acid Prevents Endothelial Dysfunction in HUVEC-12 Via Up-regulation of Lysyl Oxidase,R543.5
  8. Effect of Etiasa on the Expression of MMP-2 and TNF-α in Rat Model of Ulcerative Colitis,R574.62
  9. Fucosylation Influences Extracellular Matrix Accumulation in Tgf-β-stimulated Hk-2 Cells,R692
  10. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576
  11. Retinoic acid and matrine intervention rat hepatoma change process β-catenin, E-cadherin, MMP-7 dynamic changes,R735.7
  12. Rhubarb , magnesium sulfate treatment of severe acute pancreatitis comparative study of the clinical efficacy,R576
  13. Analysis on Effects of Expression of MMPs and ECM by Hormonal and Correlation of β1,4-GalTⅠ,R363
  14. Meningiomas and MMP-9, VEGF expression and tumor angiogenesis and recurrence,R739.45
  15. Effect of Rosuvastatin on Expression of MMP-9 mRNA and Protective in Cultured Human Monocyte-macrophages Cell Induced by Oxidized Low Density Lipoprotein,R965
  16. Protective Effect of Edaravone in the Intestinal Mucosal Barrier Injury of Severe Acute Pancreatitis in Rats,R965
  17. Atorvastatin Calcium on the Rat Carotid Artery Intimal Hyperplasia After Balloon Injury and the Expression of MMP-2,R965
  18. The Research on MMP-10 Expressing in Human Benign and Malignant Breast Tissue,R737.9
  19. The Expression and Clinical Significance of CD147 in Human Bladder Transitional Cell Carcinoma,R737.14
  20. Experiment Study of Wnt/β-catenin Signaling Pathway in the Pathogenesis of Osteoarthritis,R684.3
  21. Expressions of Matrix Metalloproteinase-3 and Matrix Metalloproteinase-9 in the Serum and Sacroiliac Joint of Spondyloarthropathy,R593.2

CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Pancreas > Pancreatitis
© 2012 www.DissertationTopic.Net  Mobile