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Purpose: the tissue microarrays group (Tissue Micro array Group TMAG) and the nucleus microarray (Nucleus Microarray NMA) technology, immunohistochemistry (Immunohistochemistry IHC) and fluorescence in situ hybridization (Fluorescence in situ hybridization FISH) detection of breast cancer tissue of HER-2 gene status and protein expression, the observed relationship between two test results and clinical significance. Materials and methods: 1. Research material collected in the General Hospital of Shenyang Military Region, June 2006 to August 2008, a clear diagnosis of breast cancer specimens, a total of 248 cases were female, aged 31 to 84 years, an average of 48.3 years. The 2 production TMAG and IHC staining different sources (Tissue microarray TMA) slices arranged in the same one on the slide, while narrowing the distance of adjacent tissue core, increasing the quantity of the tissue core, made TMAG. S-P method for IHC. IHC staining results with reference to U.S. FDA standards: the light microscope membrane brownish yellow staining positive divided into IHC-~~. Judged as positive by IHC - to level staining negative. Production NMA and FISH staining of paraffin-embedded tissue thicker slices, after dewaxing and digest, extract intact nuclei. Extracting the nucleus suspension infusion in prefabricated paraffin die hole, and then nuclei fixed the dewaxing then fabricated nucleus microarray. NMA preferably suitable for fluorescence in situ hybridization staining. FISH probes mixed probe mark throughout the HER-2 gene sequence for the orange fluorescence; another marker chromosome 17 centromere gene sequence for green fluorescence. The test results are listed the 20 interphase nuclei with HER-2 copy number centromere ratio, breast cancer HER-2 amplified cases fluorescent orange and green fluorescence signal ratio greater than or equal to 2.0; normal nucleus two signal ratio is less than 2.0; suspicious results should be regarded as the critical point (1.8 to 2.2). 4. Statistical analysis of the results of the two methods detect correlation analysis using the SPSS 11.5 statistical package χ ~ 2 test. Results: IHC detected 248 cases of breast cancer specimens, IHC-90 cases, IHC, 49 cases, IHC, 37 cases, IHC 72 cases. According to the criteria of the IHC results positive in 72 cases, negative in 176 cases, the positive rate was 29.0%. FISH test results in 248 cases of breast cancer pathology specimens, FISH negative 172 cases, FISH positive in 76 cases, FISH-positive rate of 30.6%. Results of 3.IHC with FISH in 72 cases of IHC, 59 were FISH positive; the IHC - ~ 176 specimens, 159 cases FISH negative. IHC and FISH results total coincidence rate was 87.9%, the presence of significant relevance between IHC and FISH test results. IHC and FISH results was no significant correlation (P gt; 0.01). 4.17 chromosome haplotype chromosomes and polyploid IHC four cases of chromosome 17 haplotype chromosomes, the FISH detection are positive FISH 5.26% of the total number of positive cases. IHC in five cases of polyploid chromosome, FISH chromosome 17 were negative 2.9%, accounting for the the FISH negative total number of cases. Conclusion 1. TMAG and NMA technology suitable for IHC and FISH detection of breast cancer tissue. The same having good results identity, accurate and low-cost advantages. 2 breast cancer IHC - ~ and circumstances, IHC and FISH test results are in good agreement. 3 breast cancer IHC time, need for FISH detection of HER-2 gene status, and whether to exclude the 17th chromosome haploid avoid unnecessary targeted therapy. The innovation of this study an experimental TMAG / NMA technology applications to ordinary paraffin-embedded breast cancer tissue IHC and FISH detection, expand the breast cancer detection conditions. 2 IHC cases were detected by FISH, to distinguish between the 17th staining haploid avoid unnecessary targeted drug therapy. The shortcomings of the study: In this study, not all cases of long-term follow-up, can not draw more meaningful conclusions.
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