Dissertation > Excellent graduate degree dissertation topics show

The Expression of Cortistatin in Psoriasis and the Effect of Exogenous Cortistation on a Proliferation Model of EGF-induced HaCaT Cell

Author: HeHuiLan
Tutor: ChenXiang
School: Central South University
Course: Skin and Venereology
Keywords: HaCaT cells Cortex endostatin Ki-67 EGF Proliferation Psoriasis
CLC: R758.63
Type: Master's thesis
Year: 2011
Downloads: 23
Quote: 0
Read: Download Dissertation

Abstract


The purpose of detection of cortical endostatin expression in plasma and skin of patients with psoriasis vulgaris (CST), and the application of exogenous CST HaCaT cell proliferation observed in HaCaT cell proliferation induced by epidermal growth factor (EGF) model, discussed the role of the CST in the pathogenesis of psoriasis. The method 1.ELISA assay CST expression in the plasma of 72 patients with psoriasis vulgaris and 76 cases of normal control group, immunohistochemical method detected 14 cases of psoriasis patients (from the 72 cases) lesional and normal CST's expression in control skin. 2.1 × 106 / holes HaCaT cells were seeded in six-well plates and cultured for 24 hours, the cells immunohistochemical methods to detect the HaCaT CST protein expression, RT-PCR was used to detect the expression of CST mRNA. 5000 / hole inoculated HaCaT cells to 96-well plates, RPMI-1640 containing 10 ng / ml EGF Peiji When the cells adherent for the exogenous administration of different concentrations of CST (10-9-10-6M the) processing containing 10 ng / ml EGF HaCaT cells 24 hours, MTT assay was used to detect the change of cell proliferation, and repeated three times to observe the impact of exogenous CST EGF-induced HaCaT cell proliferation and select the best cortex endostatin concentration for subsequent experiments . 4 cell immunohistochemical methods to detect the CST and the EGF the HaCaT cell group (control group), containing 10 ng / ml EGF HaCaT cells (control group) and containing 10-6M CST and 10 ng / ml EGF HaCaT cells three groups (experimental group) of Ki-67 proliferation index changes. 5 enzyme-linked immunosorbent assay (ELISA) to detect changes in the three groups of second messenger cAMP. Results 1. The psoriasis patient plasma and skin lesions in the cortex endostatin expression than the normal control group were significantly lower (P lt; 0.05) but the expression levels of the psoriasis patient plasma CST PASI score no significant correlation (P gt; 0.05). The 2.CST protein in HaCaT cytoplasmic expression. And CST mRNA (173 bp) expression in HaCaT cells. 3 different concentrations CST (10-9-10-6M) in a concentration-dependent inhibition of 10 ng / ml EGF-induced proliferation of HaCaT cells. 10-6M best inhibitory effect, the inhibition rate of 54.62% (P values ??lt; 0.05) 4.Ki-67 expression in HaCaT cells of 10 ng / ml EGF treatment group (control group) increased more blank group 10-6M CST treatment, Ki-67 were down-regulated (P = lt; 0.05). 5 second messenger cAMP and Ki-67 change (P = lt; 0.05). Conclusion (1) patients with psoriasis vulgaris lesions and plasma CST than the normal control group decreased expression may be involved in the pathogenesis of psoriasis. (2) exogenous the CST can inhibit EGF-induced proliferation of HaCaT cells.

Related Dissertations

  1. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  2. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  3. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  4. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  5. Effect of Cysteamine on the Rate of Early Successful Weaning and Immunity of Calves,S823.5
  6. Antitumor Effects of Elemene on the Proliferation Inhibition and Apoptosis Induction in Ascites Hepatoma Cell Line H22,R735.7
  7. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  8. Primary Research on Strring Culture and Storing of Human Dermal Fibroblasts,R329
  9. Inhibitory Role of Plasmodium Yoelii Circumsporozoite Protein on the Proliferation and Survial of Tumor Cell,R730.5
  10. The Experimental Study of Semiconductor Laser Radiation and 5-FU Sustained Release Repressed Oral Malignant Tumor Cell,R739.8
  11. Urinary Inflammatory Myofibroblastic Tumor Clinicopathological Analysis and Literature Review,R737
  12. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  13. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  14. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  15. Effect of Bufalin on Proliferation and Induce Apoptosis of Vascular Endothelial Cells EAhy926,R285
  16. Effect of Esculentoside a on Apoptosis and Expression of PCNA、Caspase-3、Fas and FasL in Renal Tissue of BXSB Mice,R285.5
  17. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  18. Down-regulation of ROCK Isoforms on Migration and Proliferation of Vascular Smooth Muscle Cells Stimulated by PDGF,R363
  19. Effects of Arsenic Trioxide on the Proliferation, Apoptosis and the Expression of BMP-2 Gene in Osteoblasts Cultured in Vitro,R363
  20. Research of Liver Stem Cells Proliferation after Partial Hepatictomy,R657.3
  21. Maximum Tolerated Dose Combined with Metronomic Chemoterpy on Proliferation and Angiogenesis in Treatment of Breast Cancer,R737.9

CLC: > Medicine, health > Dermatology and Venereology > Dermatology > The reason is unknown skin disease > Psoriasis (psoriasis)
© 2012 www.DissertationTopic.Net  Mobile