Dissertation > Excellent graduate degree dissertation topics show

Effects of Quercetin on the Expresion of Microsomal Triglyceride Transfer Protein and Apoprotein B in Human Hepatocytes.

Author: HeJuan
Tutor: YangXueFeng
School: Nanhua University
Course: Internal Medicine
Keywords: quercetin human HL-7702 hepatocyte steatosis trigelyceride mirosomal triglyceride transfer protein apoB
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 82
Quote: 0
Read: Download Dissertation

Abstract


Objectives: To observe the effects of quercetin on triglyceride levels , and the expression of apoprotein B(apoB) and mirosomal triglyceride transfer protein(MTP) levels in human HL-7702 steatosis hepatocytes. To study the pathogenesis of steatosis hepatocytes and the prevent effects of quercetin.Methods: Be used HL-7702 hepatocytes of normal human as study objects.and the hepatocytes were under fatty change after being exposed to RPMI-1640 complete medium with 10% (V/V) fetal bovine serum plus 10% (V/V) medical fat emulsion injection for 24 hours . we observed the cellular morphology and the formation of lipid droplets in these cells by oil red O staining, and detected the intracellular amount of triglyceride levels through high performance liquid chromatography (HPLC), Then we detected the ApoB and MTP mRNAs’expression of these hepatocytes by the semiquantitative RT- PCR. These hepatocytes were divided into 7 groups: the first group were normal hepatocytes; the second group were fatty changed hepatocytes; from group 3 to group 5, different amount of quercetin was added to those fatty changed hepatocytes to make the concentration of quercetin to be 20μmol/L、40μmol/L、80μmol/L respectively; Group 6 was the positive control group, i.e Gemfibrozil was added to those fatty changed hepatocytes to make the concentration to be 40μmol/L;In group 7 quercetin was added to the normal hepatocytes to make the concentration to be 40μmol/L. All these groups were incubated for another 24 hours,. We observed the cellular morphology and the formation of lipid droplets and detected the intracellular amount of triglyceride levels in these cells, and we detected the ApoB and MTP mRNAs’expression of these hepatocytes .Then we exposed the fatty changed cells to quercetin at the concentration of 40μmol/L for 12h、24h and 48h respectively, and we observed the cellular morphology and the formation of lipid droplets and detected the intracellular amount of triglyceride levels in these cells, and we detected the ApoB and MTP mRNAs’expression of these hepatocytes. All experimental datas were expressed as±s, All statistical analyses were performed by using the SPSS13.0 software. A P value of <0.05 was considered to be of statistical significance.Results: Be exposed normal hepatocytes to RPMI-1640 complete medium with 10% (V/V) fetal bovine serum plus 10% (V/V) medical fat emulsion injection for 24 hours , By Inverted microscope we could find out numerous lipid droplets in the cytolymph of these cells ,which could be dyed to be red by oil red O. what’s more intracellular TG level increased dramatically through measurement of HPLC(P﹤0.001). Then we exposed the steatosis hepatocytes to various concentrations of quercetin for 24 hours. By oil red O staining the result indicated that the intracellular lipid droplets of steatosis hepatocytes were much less than before, and quercetin decreased the intracellular TG levels of steatosis hepatocytes in a dose-dependent manner which could be detected by HPLC.What’s more, quercetin enhanced the expression of apoB and MTP in a dose-dependent manner which could be detected by the semiquantitative RT- PCR; Then be exposed the steatosis hepatocytes to quercetin of 40μmol/L for 12h、24h and 48h, the result indicated that quercetin decreased the intracellular TG levels of steatosis hepatocytes and induced the up-regulated expression of apoB and MTP in a time-dependent manner .Conclusions:1. Quercetin may decrease the content of intracellular trigelyceride in the human HL-7702 steatosis hepatocytes.2. Quercetin may enhance the mRNAs’expression of apoB and MTP.

Related Dissertations

  1. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  2. Some Fluorescence Quenching Spectral Analysis Interacted with Bio-related Molecules,X832
  3. Effects of Quercetin on Human Bladder Cancer Cells (EJ) and STAT3 Signaling Pathway,R737.14
  4. Taxifolin , quercetin on LPS-induced abortion mouse macrophages affect the uterus,R285.5
  5. Effect of Quercetin/hemeoxygenase-1/bilirubin System on Ethanol-induced Injury of Rat Hepatocytes,R285.5
  6. Immunosensor microfluidic chip - based research,R541.4
  7. Quercetin on SW480 human colorectal cancer cell invasion and metastasis in vitro,R285
  8. Study on Preparation of Quercetin from Buckwheat by Microbial Fermentation,TQ461
  9. Geranylgeranylacetone Alleviates Cecal Ligation and Puncture Sepsis-induced Organ Injury in Rats,R459.7
  10. The Reversion Effect on Esophageal Cancer Cell Malignancy by Liposomal Quercetin,R735.1
  11. The Apoptosis Effects on Human Esophageal Cancer Cells by Soyasaponin Bb and Nanoliposomal Quercetin,R735.1
  12. Study on Nano-Crystalline Suspension of Quercetin,R283
  13. Synthesis, Characterization and Preliminary Application of Several Organic Chromium Complexes,O627.63
  14. Effect of Carotenoids and Quercetin on K562 Cell Proliferation and PPARγ Protein Expression,R733.7
  15. Study on the Mechanisms of Quercetin and Kaempferol Induced Apoptosis in the Human Leukemic Cells,R733.71
  16. Study on Chemical Constituents of Herba Bupleuri,R284
  17. Effect of Dynamic High Pressure Microfluidization on Extraction, Structure and Antioxidant of Flavonoids from Sweet Phtato Leaves,TQ914.1
  18. The Research of Interaction and Mechanism between Molecular Drug and Biological Macromolecules,R96
  19. Preventive and Therapeutic Effects of Three Anti-oxidants on Hyperuricemia in Rats,R589.7
  20. Expressed of Visfatin and Retinol Binding Protein 4 in the Non-alcoholic Fatty Liver Tissue of Rats,R575.5
  21. Mechanism of Hepatic Steatosis Induced by HCV core Protein,R575.5

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile