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Objective: To observe the effect of Yinchenchengqi Decoction on severe acute pancreatitis in rats coagulation function of traditional Chinese medicine treatment of severe acute pancreatitis (severe acute pancreatitis, SAP) provides a theoretical basis and new treatment ideas. Methods: 30 healthy male SD rats were randomly divided into three groups: control group; model group; Chinese medicine treatment group (abbreviated CMB); n = 10. Model the steps of: (1) anesthesia: with 4% sodium pentobarbital at a dose of 1ml/kg body weight by intraperitoneal injection of anesthesia. (2) after the commencement of anesthesia, the surgical board fixed rats, supine position, cut the abdomen rat hair, routine disinfection, shop towels ready for surgery. (3) take the abdominal incision about 3cm, under strict aseptic abdominal cavity was opened to find the gastroduodenal junction, then down to find the pancreatic bile duct openings. (4) with arterial occlusion clamp hepatic duct, the 4.5-gauge needle flat side up in the opening line of pancreatic duct duodenum subcapsular puncture, and to promote pancreatic direction about 2-3mm, weight will be a press 1ml/kg % sodium deoxycholate retrograde bolus to the pancreatic duct (0.2ml/min), so that the entire pancreatic duct uniform uplift, withdraw the needle, the needle at about oppression by hand 3-5min, then remove the arterial clamp, the abdomen, and simultaneously begin timing observation. The control group was sham-operated group; model group used by the pancreatic duct to rat pancreatic tissue by retrograde injection of 1% sodium deoxycholate method induced severe acute pancreatitis (SAP), traditional Chinese medicine group and the model group using the same method Manufacturing SAP rats, and were in before modeling 12h and 12h after modeling capillaris oral gavage medicine decoction decoction liquid 2ml / g. Modeling after 24h blood, blood tests prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and international normalized ratio (INR), the test results for each group of rats pairwise Comparison. Results: 2.1 PT: PT mild extension model group compared with the control group, the difference was not significant, was not statistically significant (P gt; 0.05), treatment group compared with the model group PT has been shortened, the difference was not significant, does not have the statistically significant (P gt; 0.05), treatment group compared with the control group, the difference was not significant, was not statistically significant (P gt; 0.05). 2.2 APTT: mild prolonged APTT model group compared with the control group, the difference was not significant, was not statistically significant (P gt; 0.05), treatment group compared with the model group APTT has been shortened, the difference was not significant, was not statistically significance (P gt; 0.05), treatment group compared with the control group, the difference was not significant, was not statistically significant (P gt; 0.05). 2.3 FIB: model group FIB was significantly higher in the control group was significantly different, with statistical significance (P lt; 0.01), treatment group compared with model group FIB significantly reduced, significantly different, with statistical significance (P lt; 0.01 ), but still higher in control group (P lt; 0.01). 2.4 INR: model group, INR values ??increased slightly, compared with the control group, the difference was not significant, was not statistically significant (P gt; 0.05), treatment group compared with the model group, no significant change in INR, the difference was not statistically significant ( P gt; 0.05), treatment group compared with the control group, the difference was not significant, was not statistically significant (P gt; 0.05). Conclusion: Herbal capillaris Dachengqitang can effectively reduce the blood of rats with severe acute pancreatitis fibrinogen (FIB) content, improve severe acute pancreatitis in rats coagulopathy severity for the clinical treatment SAP provides a theoretical basis and new ideas ; severe acute pancreatitis in rats fibrinogen (FIB) was significantly higher in a short time, prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) is not within 24h significant changes; medicine decoction capillaris severe acute pancreatitis in rats prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) had no significant effect in a short time.
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