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A Quantitative Study on Early Demineralization of Bovine Enamel Based on Optical Coherence Tomography

Author: XuYanYong
Tutor: YaoZuo
School: Tianjin Medical University
Course: Biomedical Engineering
Keywords: Bovine permanent teeth Enamel Early demineralization Quantitative research Optical CT technology
CLC: R783.5
Type: Master's thesis
Year: 2011
Downloads: 35
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Abstract

Objective: The the early caries occurrence of the enamel surface demineralization and remineralization imbalance caused by caries bacteria produce acid, and eventually lead to tooth partial de-mine changes. Therefore, the quantitative study of enamel demineralization early to accurately quantify the necessary means for the diagnosis of early caries. In this study, the optical CT on early artificial bovine permanent teeth enamel demineralization in vitro quantitative research, using atomic absorption spectrometry determination of the amount of calcium lost for different times of the day off mine to be verified as to its accuracy, aimed at exploring an accurate quantitative analysis of enamel The new method of early demineralization, further oral clinical application of optical CT quantitative diagnosis and monitoring of early caries provide a scientific basis and experimental data. Methods: bovine permanent teeth samples taken from the Experimental Research Center of Tianjin Stomatological Hospital vitro tooth sample library, are within two weeks of freshly collected, and do go to the root to the marrow processing, to retain the crown. The following experiments and data analysis Experimental Research Center of Tianjin Stomatological Hospital and completed. Early bovine enamel artificial gradient de-mine model for the establishment of: screening out no chalk variable pigmentation, cracked normal away from the body bovine permanent tooth crown 10 Mei, the use of chemical de-mine law in cattle constant enamel surface preparation of different periods (24h, 48h , 72h, 96h) off the mine as an experimental window, not off mine (Oh) as a control window; chemical demineralization solution to collect and save each period and Demineralization window. Optical CT system scan imaging of experimental samples: optical CT scan imaging along the short axis of the tooth above bovine permanent teeth enamel surface experiments windows and control windows, every single bovine permanent teeth for five two-dimensional optical CT section The image, the image interval of 1500 microns. 3. Various periods experiment window gray value calculation of the amount of change in the optical CT image: (1) at various time intervals experimental window demineralization depth determined: using Matlab software control through the analysis of the comparative optical CT image in each window and the corresponding image of the experimental The average grayscale value of the window changes to determine the depth of the different off the ore degree experimental window demineralization. (2) a specific depth within the calculation of the amount of change of the gray value of each experimental window: demineralization depth, calculate the corresponding experimental window the amount of change of the gray value of each experimental window according to the above identified, i.e. the demineralization depth calculation using the determined respective experimental window gray value, subtracting the gray scale value of the control windows in the same depth within the normal enamel, to obtain the change amount of the gradation value of a particular depth within the scope of the optical CT image of each experimental window. 4 chemical de-Determination of the leaching of ore fluid experiments windows calcium: flame atomic absorption spectrometry determination of different times of the experimental window chemical off the calcium content of the ore fluid, and then subtracting the starting chemical off the calcium content in the ore fluid obtained Calvary period experimental window chemical off the calcium leaching of the ore fluids that the experimental window each period the amount of calcium lost. 5, the study of the demineralization experiment window gray value changes in the amount of calcium lost the amount of correlation: Pearson correlation analysis of different demineralization period the experimental window grayscale value changes in the amount of calcium lost. Results: 1. Early bovine enamel artificial gradient demineralization model of morphological evaluation: observed, the the 24h period early Demineralization window with normal enamel control window was no significant difference, other gradient experiments windows with off increase in the time of mineralization, chalk variable severity, expand coverage; optical CT images can clearly show the difference between each gradient demineralization experiment window with normal enamel can be observed to grayscale with de-mineralization time increases. 2. Different amount of gray value changes de-mine period experimental window: optical CT images of different de-mining period experimental window of gray value changes do one-way ANOVA analysis found that the difference was statistically significant (P lt; 0.05), and do pairwise comparison further, that with the de-mine time increase the experimental window gray value changes increased the image gray value is directly proportional to the duration of action of the demineralization. 3. Different de-mine experimental window calcium hours lost: atomic absorption spectrometry measured various degree of demineralization experiment window calcium loss amount to do single-factor analysis of variance, and the difference was statistically significant (P lt; 0.05), further do pairwise comparisons, that extend off mine calcium loss increased with the de-mineralization time. 4 different the demineralization period experimental window gray value change amount of calcium lost amount: The results showed different degree of demineralization experiment window gray value of the amount of change in the amount of calcium lost to a positive correlation (P lt; 0.05), indicating that the different de- ore extent experimental window gray value variation amount increases with the increase in the amount of calcium lost. Conclusion: 1, using optical CT image gray value changes as a parameter indicators, quantitative its different demineralization extent de-mining period experiment window for the optical CT quantitative enamel demineralization early to explore a new quantitative indicators. That early enamel off mine optical CT image gray value changes and mineral chemistry of the amount of lost relevance; the optical CT can be achieved early, sensitive, non-invasive detection bovine enamel demineralization early; image gray The amount of change as a new means of quantitative analysis, the correct experimental deviation, improve analytical methods, is expected to make enamel early caries quantitative diagnosis more accurate.

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