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pEGFP/Ang-1 Transfected Rat BMSCs Transplantation on Diabetic Retinal Vascular Leakage of Retinal

Author: ZhangKeJian
Tutor: LiuXueZheng
School: Liaoning Medical
Course: Human Anatomy and Embryology
Keywords: Diabetic retinopathy Bone marrow mesenchymal stem cells Evans blue Angiopoietin-1
CLC: R774.1
Type: Master's thesis
Year: 2011
Downloads: 43
Quote: 0
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Abstract


Pichia vascular Objective to generate prime recombinant plasmid (pEGFP/Ang-1) transfected rat of BMSCs , will be under the the transfected BMSCs line retinal transplantation , thus confirming the angiotensin 1 (Ang-1) transfected BMSCs on protective effect of the retina of diabetic rats . Methods Male SD rats 4 to 6 , the femur and tibia removed under sterile conditions , cut epiphyseal end repeated washing with medium bone marrow cavity separation of BMSCs , combined with its repeated attachment method further purified and identified . The Pichia angiopoietin recombinant plasmid (pEGFP/Ang-1) transfected rat of BMSCs after transfection Ang-1/BMSC subretinal transplantation . Replacement of 96 SD rats were randomly divided into normal rat group ( CON ) , blank control diabetes group (DM1), the retina of diabetic rats transplanted BMSCs group ( DM2 ) , the retina of diabetic rats transplanted Ang-1/BMSCs group (ANG). Tail vein injection of streptozotocin (STZ) diabetic model , molded DM2 group subretinal transplantation of BMSCs the ANG group retinal transplant Ang-1/BMSCs, respectively, 4w , 8w , 12w animals were sacrificed . With the amount of Evans blue was used to detect the infiltration of the retina ; observed the distribution integration in the context of the transplanted cells in the retina by immunohistochemistry and Western blot detection of rat retinal expression of Ang-1 . Results the DM1 the DM2 group of diabetic rats infiltration capacity and permeability gradually increases with time ; the ANG group retina transplant Ang-1/BMSCs able significantly inhibit this change . Immunohistochemical analysis confirmed the transplant Ang-1/BMSCs integration in diabetic rat retinal ganglion cell layer and inner granular layer . Western blot analysis proved DM1, DM2 group with the development of diabetic retinopathy , diabetic rat retina of Ang-1 expression was significantly reduced , while the expression of Ang-1 higher than the ANG group DM1 , DM2 increased significantly with the CON DM1, DM2 group difference was statistically significant ( P lt; 0.05 ) , DM1 and DM2 group difference was not statistically significant ( P gt ; 0.05 ) . The subretinal Conclusions 1. Ang-1/BMSCs see integration of transplanted cells in the ganglion cell layer and inner granular layer after transplantation . 2. Ang-1/BMSCs subretinal transplantation can improve the diabetic rat retina microcirculation .

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CLC: > Medicine, health > Ophthalmology > Retina and optic nerve diseases > Retinal disease
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