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The Expression and Significance of SUMOylation in Insulin-like Growth Factor Ⅰ Receptor in Acute Myeloid Leukemia

Author: ZhangJian
Tutor: ChenFangPing
School: Central South University
Course: Department of Hematology
Keywords: Acute myeloid leukemia HL-60 cells Insulin-like growth factor I receptor SUMOylation UBC9
CLC: R733.71
Type: Master's thesis
Year: 2011
Downloads: 15
Quote: 0
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Abstract


Background and Objective: In recent years, because of the insulin-like growth factor I receptor (IGF-Ⅰ R) characteristics of the tumor cell proliferation , apoptosis and metastasis , gradually become a research focus for targeted cancer therapy . Some scholars have found that the transmembrane protein IGF-Ⅰ R called SUMOylation modification , into the nucleus , suggesting that this may be its role in tumor -related . In this study, by observation of acute myeloid leukemia (AML) , IGF-Ⅰ R SUMOylation its proliferation and apoptosis of HL-60 cells , to explore the significance of targeting anti - IGF-Ⅰ R as a basis for the treatment of acute myeloid leukemia . Methods : ( 1 ) select the seven cases of acute myeloid leukemia patients , 10 cases of normal healthy control group . ( 2) develop the original acute myeloid leukemia cell line ( HL -60 ) . (3) flow cytometry to detect cell surface expression of IGF-Ⅰ R . (4) Immunocytochemistry detection cell IGF-Ⅰ R and SUMO protein co-expression . ( 5 ) inhibition of SUMOylation ligase ( UBC9 ) is , CCK8 assay cell proliferation , apoptosis , Annexin V- FITC PI double staining , the Immunocytochemistry detect IGF-Ⅰ R and SUMO proteins co-expressed . The results : ( 1 ) HL - 60 cell surface expression of IGF-Ⅰ R level was significantly higher than the normal control group (P lt; 0.05). (2) HL-60 cells and AML patients with bone marrow mononuclear cell nucleus memory a large number of co-expression of IGF-Ⅰ R SUMO protein fluorescence positive - strong percentage of positive cells ( 74.17 2.90 ) % and ( 64.79 , 12.14 ) % . In normal human peripheral blood mononuclear cell nucleus , and this co-expression of almost no . ( 3 ) through inhibition of UBC9 24 hours , 48 hours and 72 hours , cell proliferation was inhibited , the dose effect ( P lt ; 0.05 ) and time-effect relationship ( P lt; 0.05 ) , but apoptosis in a dose without significantly change (P gt; 0.05), aging , a slight change (P lt; 0.05). IGF-Ⅰ R in HL-60 nucleus of 48 hours after the suppression UBC9 and SUMO expression was significantly decreased ( 39.29 8.52% ) . Conclusion : IGF-Ⅰ R in the the AML expression of increased and cell memory SUMOylated of the receptor . Inhibit UBC9 AML within the nucleus of IGF-Ⅰ R SUMO protein 's expression significantly reduced cell proliferation also by inhibiting Tip of IGF-Ⅰ R SUMOylation referral guide of the receptor to enter the cell nucleus , and cell proliferation was positively correlated .

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia
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