Dissertation > Excellent graduate degree dissertation topics show

Expression and Significance of K17、7、10/13、8 and Ki-67 in Cervical Squamous Intraepithelial Lesion and Immature Squamous Metaplasia

Author: LianLingZhi
Tutor: HuiJing
School: Tianjin Medical University
Course: Pathology and Pathophysiology
Keywords: CIN cervical immature squamous metaplasia high-grade squamous intaepthelial lesion differentiation keratin Ki-67
CLC: R737.33
Type: Master's thesis
Year: 2011
Downloads: 34
Quote: 0
Read: Download Dissertation


Objective:Cervical cancer is the second most common cancer among women worldwide.Among them, the squamous cancer is more happened, cervical intraepithelial neoplasia is precancerous change of Cervical cancer.The difference between high risk chanciness and low risk chanciness is hard to distinguish. Keratins are a highly diverse family of intermediate filament and important markers of epithelial cell differentiation.. The composition of keratin pairs varies depending on cell type, differentiation status and environment,and a lot of researcher pay close attention to it for the auxiliary diagnosis of pathological changes. Our target was to explore the expression of keratins and Ki-67 in Cervical epithelial nonmalignant changes and tumorous changes, investigating the different of cell differentiation among Cervical epithelial changes and the function of keratins and Ki-67 as auxiliary diagnosis of pathologic diagnosis.We investigated the 2006 to 2009 formalin-fixed paraffinembedded cervical specimens:cervical punch biopsies, knife cone and loop electrosurgical excision procedure (LEEP) excision specimens with varying proportions of normal cervical squamous epithelium(n=10), immature squamous metaplasia(n=30), low-grade squamous Intraepithelial lesion(CINⅠ,n=30) and high-grade squamous intraepithelial lesion (CINⅡ=11,CINⅢ= 19) from the department of Pathology in the General Hospital in Tianj in Medical University. The expression of keratin 17、7、8、10/13 and Ki-67 were examined by immunohistochemical technique. The data was analyzed by SPSS software.Results:1.K17 was high expressed in cervical immature squamous metaplasia and low in normal cervical squamous epithelium、low-grade squamous intraepithelial lesion and high-grade squamous intraepithelial lesion.There was a significant difference between immature squamous metaplasia and high-grade squamous intraepithelial lesion P<0.01) 2. There was a significant difference of the expression of K7 among each group, K7 expression in high-grade squamous intraepithelial lesion was higher than immature squamous metaplasia、low-grade squamous intraepithelial and normal cervical squamous epithelium,in the immature squamous metaplasia was higher than low-grade squamous intraepithelial, in the low-grade squamous intraepithelial was higher than normal cervical squamous epithelium (P<0.01)3.There was a significant difference of the expression of K10/13 among each group, K10/13 expression in high-grade squamous intraepithelial lesion was lower than immature squamous metaplasia、low-grade squamous intraepithelial and normal cervical squamous epithelium (P< 0.01), there was no statistic difference of K10/13 between low-grade squamous intraepithelial and normal cervical squamous epithelium (P>0.05);4. There was no statistic difference of K8 among every group (P>0.05);5. The expression of Ki-67 in high-grade squamous intraepithelial was higher than in others (P<0.01)6. In normal cervical squamous epithelium、low-grade squamous intraepithelial and high-grade squamous intraepithelial, there were positive correlation between the expression of K7 and K10/13 (r=-0.427,P<0.001)Conclusions:1.K17 was high expressed in immature squamous metaplasia,that means it maybe the "stress" protein when cervical inflammation,while had no significantly changed in malignant disease,so K17 was useful to distinguish them;2.K7 was up-regulated with the grade of disease,while K10/13 was converse.The change of them may means the cell differentiation disordered and may be the consequence of genetype mutation;3.There had no changed of K7、10/13 expression in cervical immature squamous metaplasia,the cell was well differentiated;4. Ki-67 was helpful to diagnose hyproliferation disease;5. Atypical immature metaplasia is a group of heterogenicity disease,with the correlation between immature squamous metaplasia and high-grade squamous Intraepithelial lesion,coexpression of K17、7、10/13 and Ki-67 can distinction the innocent change from malignant disease.

Related Dissertations

  1. Exoression of Resistin During Porcine Preadipocyte Differentiation and Its Infiuencing Factors,R587.1
  2. Professor Wang Qi identified the body - of Diseases - dialectical combination of academic thought and clinical experience and treatment of chronic insomnia clinical studies,R249.2
  3. miRNA-23a into the regulation of muscle differentiation in C2C12 cells,Q952.5
  4. Evaluation of Land Use Eco-security in Fujian Province,X826
  5. Effects of Isoprocarb Exposure on Differentiation, Migration and Neurite Outgrowth of the Key Cell During Neural Development in Vitro,R329
  6. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  7. Genetic Diversity, Population Sturucture and Gene Flow of Helicoverpa Armigera (H(?)bner) in China,S435.622.3
  8. Clinical Study on Relationship Among Syndrome Differentiation Clssified in Tcm,ambulatory Blood Pressure Monitoring and Transcranial Doppler in Essential Hypertension,R259
  9. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  10. Differentiation Capacity of Periodontal Ligament Stem Cells of Chronic Periodontitis Patients with and Without Type 2 Diabetes Mellitus,R587.1
  11. Guangzhou Daily Newspaper Group, the industry value chain research,G219.24
  12. Based Capital Perspective outdoor new media strategic research and case studies,G206-F
  13. The Inhibitory Effects of Celastrol on the Process of Brain Glioma Stem Cells’ Differentiation into Vascular Endothelial Cell-like Cells,R739.4
  14. Dilemma of Low Quality Competition Strategy and Its Way Out,F273.2;F224
  15. Histopathology Result and the Significance of ACS-US,R737.33
  16. The Influence of Exogenous Hyaluronic Acid on Chondrogenic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells,R329
  17. Effects of Electroacupuncture on Differenraition of Nerve Stem Cells in Rats with Cerebral Ischemia,R245
  18. HPV16E6 cervical squamous cell carcinoma and STAT3 protein expression and correlation,R737.33
  19. Osteogenic Differentiation of mMSCs on the Biomimetic Nanofibrous Scaffolds of Hydroxyapatite/Chitosan,R318.08
  20. Induced Differentiation Suppresses the Stem Cell Porperties and Tumorigenesis of Stem-like Glioma Cells,R739.4
  21. Extraction of wool keratin and its application,TS195.56

CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
© 2012 www.DissertationTopic.Net  Mobile