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The Influence of Xinmailong on Hypoxia-inducible Factor-1α Expression after Asphyxia in Neonatal Rats

Author: HuangZuoXin
Tutor: WuXingHeng
School: Nanchang University
Course: Paediatric
Keywords: Xinmailong Hypoxic-ischemic myocardial injury Hypoxia-inducible factor -1α Endothelin-1
CLC: R722.1
Type: Master's thesis
Year: 2009
Downloads: 42
Quote: 0
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Abstract


Purpose. Atmospheric suffocation model of newborn rats, observed changes and their asphyxiation rat myocardial hypoxia inducible factor-1α (HIF-1α) and plasma endothelin -1 (ET-1) correlation. 2. Observed Xinmailong (Xinmailong) asphyxia neonatal myocardial hypoxia-inducible factor-1α and plasma endothelin-1, to investigate the hypoxic-ischemic injury on myocardial protection mechanisms. Method 1. The newborn rats atmospheric suffocation model making and grouping: newborn 7d old SD rats 90 (weight 10g to 15g) completely randomized into control group (n = 30), asphyxia group (n = 30), The Xinmailong group (n = 30); each subdivision 6h, 24h, 72h three time points, 10 each for each time point. Control group: only simulated atmospheric suffocation process, do not plug the cork reoxygenation; asphyxia group: production of atmospheric suffocation model; the treatment group Xinmailong: making atmospheric asphyxia, suffocation before 5min given Xinmailong 5mg/kg intraperitoneal injection. 2 was observed in each group the number of mice died; detection of cardiac enzymes CK, observe the morphological changes in myocardial tissue; radioimmunoassay of serum endothelin-1; immunohistochemical method detection rat myocardial hypoxia-inducible factor-1α. Statistical methods: factorial analysis, SNK-q test and Pearson correlation analysis. Results deaths each group of mice: the control group no death rat, asphyxia group, four died, one died while Xinmailong group. 2. Cardiac enzyme CK: no significant change in the control group at each time point CK concentration; asphyxia group elevated CK levels at 6h, peaked at 24h, 72h decline, at each time point was significantly higher (P lt; 0.01); Although heart pulse Long of CK values ??at each time point was significantly higher (P lt; 0.01), but significantly lower than the same period asphyxia group (P lt; 0.01). 3 plasma ET-1 level: the control group at each time point no significant changes in plasma ET-1 levels; the asphyxia group ET-1 values ??in 6h rise peaked, 24h, 72h decline, significantly higher than the control group at all time points (P lt; 0.01); ET-1 the though Xinmailong group values ??at each time point was significantly higher (P lt; 0.01), but significantly lower than the same time period asphyxia group (P lt; 0.01). Myocardial hypoxia inducible factor-1α immunohistochemistry optical density values: no significant change in the control group at all time points HIF-1α optical density value; the the asphyxia group HIF-1α light density value at 6h elevated, peaked at 24h, 72h decline significantly higher than that of the control group at all time points (P lt; 0.01); HIF-1α the though Xinmailong group optical density values ??significantly at all time points higher than that of the control group (P lt; 0.01), but significantly lower than the same period of time choking group (P lt; 0.01). Myocardial histopathology: control group neonatal myocardial neat close, no edema in the organization, nucleus Dense clear; asphyxia group compared Xinmailong myocardial cell edema, infiltration of inflammatory cells to reduce cell necrosis. 6. The asphyxia group HIF-1α expression and plasma ET-1 levels were positively correlated (r = 0.876, P lt; 0.01). Conclusion 1. Suffocation increased of mouse serum ET-1 level and myocardial HIF-1α expression, suggesting that ET-1 and HIF-1α involved in the pathological changes of myocardial hypoxia ischemia. Prompted suffocation HIF-1α may be involved in the regulation of target gene expression of ET-1. Suffocation mouse serum of ET-1 levels and myocardial HIF-1α expression was positively correlated. 3. Xinmailong pretreatment can reduce suffocation deaths mice, reduce cardiac enzymes, alleviate myocardial ischemia pathological changes, and its role in the play may be associated with reduced myocardial HIF-1α expression and serum levels of ET-1 related.

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CLC: > Medicine, health > Pediatrics > Newborns, premature children disease > Neonatal disease
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